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Effects of Photosystem II Interfering Herbicides Atrazine and Bentazon on the Soybean Transcriptome 1

ABSTRACT: Atrazine and bentazon are both photosystem II inhibiting herbicides that interfere with photosynthetic electron transport provoking oxidative stress. While atrazine is lethal to soybean, bentazon does not kill soybeans because of the capability of soybeans to metabolize the herbicide. Gene expression profiling was conducted using cDNA microarrays to understand the responses of soybeans to PSII interruption and concomitant oxidative stress caused by atrazine and bentazon by monitoring expression at 1, 2, 4, and 8 h after treatment (HAT). The microarray study revealed that 6,646 genes were differentially expressed with high statistical significance over the experiment with 88% of them sharing similar expression pattern between the atrazine and bentazon treatments. Many genes related to xenobiotic detoxification and antioxidation such as cytochrome P450s, glutathione-S-transferases, superoxide dismutases, catalases and tocopheral cyclases were induced by the herbicides. The study also discovered plants treated with bentazon started to recover between 4 HAT and 8 HAT as reflected in the decreased amplitude of fold changes of most genes from 4 to 8 HAT. The 12% of the genes that were differentially expressed between atrazine and bentazon were largely related to cell recovery, such as genes related to ribosomal components. Keywords = stress Keywords = GST Keywords = ROS Keywords = D1 protein Keywords = Fuzzy k-means Keywords = plant Keywords: plant xenobiotic interaction loop design, 12 direct comparisons, 2 technical repeats including dye swaps, 3 biological repeats

ORGANISM(S): Glycine max

SUBMITTER: Osman Radwan

PROVIDER: E-GEOD-15374 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Effects of Photosystem II Interfering Herbicides Atrazine and Bentazon on the Soybean Transcriptome 2

Project description:Atrazine and bentazon are both photosystem II inhibiting herbicides that interfere with photosynthetic electron transport provoking oxidative stress. While atrazine is lethal to soybean, bentazon does not kill soybeans because of the capability of soybeans to metabolize the herbicide. Gene expression profiling was conducted using cDNA microarrays to understand the responses of soybeans to PSII interruption and concomitant oxidative stress caused by atrazine and bentazon by monitoring expression at 1, 2, 4, and 8 h after treatment (HAT). The microarray study revealed that 6,646 genes were differentially expressed with high statistical significance over the experiment with 88% of them sharing similar expression pattern between the atrazine and bentazon treatments. Many genes related to xenobiotic detoxification and antioxidation such as cytochrome P450s, glutathione-S-transferases, superoxide dismutases, catalases and tocopheral cyclases were induced by the herbicides. The study also discovered plants treated with bentazon started to recover between 4 HAT and 8 HAT as reflected in the decreased amplitude of fold changes of most genes from 4 to 8 HAT. The 12% of the genes that were differentially expressed between atrazine and bentazon were largely related to cell recovery, such as genes related to ribosomal components. Keywords = stress Keywords = GST Keywords = ROS Keywords = D1 protein Keywords = Fuzzy k-means Keywords = plant Keywords: plant xenobiotic interaction loop design, 12 direct comparisons, 2 technical repeats including dye swaps, 3 biological repeats

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Project description:This SuperSeries is composed of the following subset Series: GSE15374: Effects of Photosystem II Interfering Herbicides Atrazine and Bentazon on the Soybean Transcriptome 1 GSE15375: Effects of Photosystem II Interfering Herbicides Atrazine and Bentazon on the Soybean Transcriptome 2 Refer to individual Series

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Gene expression profiling soybean stem tissue early response to Sclerotinia sclerotiorum 1

Project description:White mold disease, caused by Sclerotinia sclerotiorum (Lib) de Bary, can be a serious disease of crops grown under cool moist environments. In many plants, like soybean [Glycine max (L.) Merr.], complete genetic resistance does not exist. To identify possible genes involved in defense against this pathogen, and to determine possible physiological changes that occur during infection, a microarray screen was conducted using stem tissue to evaluate changes in gene expression between partially resistant and susceptible soybean genotypes at 8 and 14 hours post inoculation. RNA from 15 day old inoculated plants was labeled and hybridized to soybean cDNA microarrays. ANOVA identified 1270 significant genes from the comparison between time points and 105 genes from the comparison between genotypes. Selected genes were classified into functional categories. The analyses identified changes in cell-wall composition and signaling pathways, as well as suggesting a role for anthocyanin and anthocianidin synthesis in the defense against S. sclerotiorum. In-silico mapping of both the differentially expressed transcripts and of public markers associated with partial resistance to white mold, provided evidence of several differentially expressed genes being closely positioned to white mold resistance markers, with the two most promising genes encoding a PR-5 and anthocyanidin synthase. Keywords = white mould Keywords = necrotroph Keywords = oxalic acid Keywords = defense Keywords = ANOVA Keywords = PI 194639 Keywords = plant Keywords: plant microbe interaction 1 comparison, 2 technical repeats including dye swaps, 3 biological repeats

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Project description:White mold disease, caused by Sclerotinia sclerotiorum (Lib) de Bary, can be a serious disease of crops grown under cool moist environments. In many plants, like soybean [Glycine max (L.) Merr.], complete genetic resistance does not exist. To identify possible genes involved in defense against this pathogen, and to determine possible physiological changes that occur during infection, a microarray screen was conducted using stem tissue to evaluate changes in gene expression between partially resistant and susceptible soybean genotypes at 8 and 14 hours post inoculation. RNA from 15 day old inoculated plants was labeled and hybridized to soybean cDNA microarrays. ANOVA identified 1270 significant genes from the comparison between time points and 105 genes from the comparison between genotypes. Selected genes were classified into functional categories. The analyses identified changes in cell-wall composition and signaling pathways, as well as suggesting a role for anthocyanin and anthocianidin synthesis in the defense against S. sclerotiorum. In-silico mapping of both the differentially expressed transcripts and of public markers associated with partial resistance to white mold, provided evidence of several differentially expressed genes being closely positioned to white mold resistance markers, with the two most promising genes encoding a PR-5 and anthocyanidin synthase. Keywords = white mould Keywords = necrotroph Keywords = oxalic acid Keywords = defense Keywords = ANOVA Keywords = PI 194639 Keywords = plant Keywords: plant microbe interaction loop design, 4 comparisons, 2 technical repeats including dye swaps, 3 biological repeats

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Effects of Photosystem II Interfering Herbicides Atrazine and Bentazon on the Soybean Transcriptome 1

2010-03-24 | GSE15374 | GEO

Effects of Photosystem II Interfering Herbicides Atrazine and Bentazon on the Soybean Transcriptome 2

2010-03-24 | GSE15375 | GEO