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Effects of Photosystem II Interfering Herbicides Atrazine and Bentazon on the Soybean Transcriptome 2

ABSTRACT: Atrazine and bentazon are both photosystem II inhibiting herbicides that interfere with photosynthetic electron transport provoking oxidative stress. While atrazine is lethal to soybean, bentazon does not kill soybeans because of the capability of soybeans to metabolize the herbicide. Gene expression profiling was conducted using cDNA microarrays to understand the responses of soybeans to PSII interruption and concomitant oxidative stress caused by atrazine and bentazon by monitoring expression at 1, 2, 4, and 8 h after treatment (HAT). The microarray study revealed that 6,646 genes were differentially expressed with high statistical significance over the experiment with 88% of them sharing similar expression pattern between the atrazine and bentazon treatments. Many genes related to xenobiotic detoxification and antioxidation such as cytochrome P450s, glutathione-S-transferases, superoxide dismutases, catalases and tocopheral cyclases were induced by the herbicides. The study also discovered plants treated with bentazon started to recover between 4 HAT and 8 HAT as reflected in the decreased amplitude of fold changes of most genes from 4 to 8 HAT. The 12% of the genes that were differentially expressed between atrazine and bentazon were largely related to cell recovery, such as genes related to ribosomal components. Keywords = stress Keywords = GST Keywords = ROS Keywords = D1 protein Keywords = Fuzzy k-means Keywords = plant Keywords: plant xenobiotic interaction

ORGANISM(S): Glycine max

PROVIDER: GSE15375 | GEO | 2010/03/24

SECONDARY ACCESSION(S): PRJNA122943

REPOSITORIES: GEO

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Effects of Photosystem II Interfering Herbicides Atrazine and Bentazon on the Soybean Transcriptome 2

Project description:Atrazine and bentazon are both photosystem II inhibiting herbicides that interfere with photosynthetic electron transport provoking oxidative stress. While atrazine is lethal to soybean, bentazon does not kill soybeans because of the capability of soybeans to metabolize the herbicide. Gene expression profiling was conducted using cDNA microarrays to understand the responses of soybeans to PSII interruption and concomitant oxidative stress caused by atrazine and bentazon by monitoring expression at 1, 2, 4, and 8 h after treatment (HAT). The microarray study revealed that 6,646 genes were differentially expressed with high statistical significance over the experiment with 88% of them sharing similar expression pattern between the atrazine and bentazon treatments. Many genes related to xenobiotic detoxification and antioxidation such as cytochrome P450s, glutathione-S-transferases, superoxide dismutases, catalases and tocopheral cyclases were induced by the herbicides. The study also discovered plants treated with bentazon started to recover between 4 HAT and 8 HAT as reflected in the decreased amplitude of fold changes of most genes from 4 to 8 HAT. The 12% of the genes that were differentially expressed between atrazine and bentazon were largely related to cell recovery, such as genes related to ribosomal components. Keywords = stress Keywords = GST Keywords = ROS Keywords = D1 protein Keywords = Fuzzy k-means Keywords = plant Keywords: plant xenobiotic interaction loop design, 12 direct comparisons, 2 technical repeats including dye swaps, 3 biological repeats

2010-03-23 | E-GEOD-15375 | biostudies-arrayexpress

Effects of Photosystem II Interfering Herbicides Atrazine and Bentazon on the Soybean Transcriptome 1

2010-03-23 | E-GEOD-15374 | biostudies-arrayexpress

Effects of Photosystem II Interfering Herbicides Atrazine and Bentazon on the Soybean Transcriptome 1

2010-03-24 | GSE15374 | GEO

Effects of Photosystem II Interfering Herbicides Atrazine and Bentazon on the Soybean Transcriptome

Project description:This SuperSeries is composed of the following subset Series: GSE15374: Effects of Photosystem II Interfering Herbicides Atrazine and Bentazon on the Soybean Transcriptome 1 GSE15375: Effects of Photosystem II Interfering Herbicides Atrazine and Bentazon on the Soybean Transcriptome 2 Refer to individual Series

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Herbicides

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Metabolism-based resistance to herbicides in black-grass

Project description:Resistance to herbicides in weeds can be due to alteration(s) in the gene encoding the herbicide target site, or to herbicide degradation via a deviation in plant general metabolism. If target-site-based resistance is easy to study, the multigenic control of metabolism-based resistance renders it much more complex to study. Metabolism-based resistance to herbicides represents the major part of herbicide resistance in black-grass. Its most likely basis is an overexpression of genes encoding enzymes degrading herbicides. We thus seek to identify such overexpressed genes by comparing the transcriptomes of resistant and sensitive black-grass individuals belonging to an F2 line segregating for two resistance genes. Given there are no genomic tools developed for black-grass, this approach will use heterologous hybridisation onto a wheat Affymetrix microarray. Comparison using heterologous hybridisation onto a wheat whole-genome microarray of transcriptome of three pools of black-grass plants obtained 2h30 after herbicide spraying at field rate. The three pools correspond to: · Sensitive plants (killed by herbicide). · Moderately resistant plants (growth impaired by herbicide but plants still alive) · Resistant plants (growth unimpaired by herbicide)

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Transcriptomic analyses reveal the effects of herbicides on the mycelium of Morchella sextelata

Project description:Morchella sextelata, recognized for its medicinal properties and distinctive taste, is a highly valued edible mushroom. Despite its commercial importance, the mushroom's susceptibility to environmental factors and its reactions to herbicides commonly used in cultivation have not been extensively studied. This research aimed to explore how four prevalent herbicides—Trifluralin, Pendimethalin, S-metolachlor, and Acetochlor—affect the mycelial growth of Morchella sextelata. The study involved cultivating the mycelium in a Potato Dextrose Agar (PDA) medium with concentrations of herbicides at ten times the standard application rate, followed by measuring the degree of mycelial growth inhibition. Employing transcriptomic analysis, RNA sequencing was conducted on the mycelium exposed to the herbicides. The subsequent transcriptome profiling indicated that the application of these herbicides impacted various biological pathways within Morchella sextelata, with a notable influence on glycolysis/gluconeogenesis and the glyoxylate cycle, which are crucial for energy production and metabolic processes. While Trifluralin and Pendimethalin demonstrated a less pronounced negative impact on mycelial growth, the study concluded that all tested herbicides were detrimental to Morchella sextelata. It is thus recommended that, in agricultural practice, the use of herbicides be approached with caution, and that application rates be strictly adhered to in order to ensure the healthy cultivation of Morchella sextelata and minimize potential pesticide residue issues.

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Metabolism-based resistance to herbicides in black-grass

Project description:Resistance to herbicides in weeds can be due to alteration(s) in the gene encoding the herbicide target site, or to herbicide degradation via a deviation in plant general metabolism. If target-site-based resistance is easy to study, the multigenic control of metabolism-based resistance renders it much more complex to study. Metabolism-based resistance to herbicides represents the major part of herbicide resistance in black-grass. Its most likely basis is an overexpression of genes encoding enzymes degrading herbicides. We thus seek to identify such overexpressed genes by comparing the transcriptomes of resistant and sensitive black-grass individuals belonging to an F2 line segregating for two resistance genes. Given there are no genomic tools developed for black-grass, this approach will use heterologous hybridisation onto a wheat Affymetrix microarray. Comparison using heterologous hybridisation onto a wheat whole-genome microarray of transcriptome of three pools of black-grass plants obtained 2h30 after herbicide spraying at field rate. The three pools correspond to: Â· Sensitive plants (killed by herbicide). Â· Moderately resistant plants (growth impaired by herbicide but plants still alive) Â· Resistant plants (growth unimpaired by herbicide) 6 arrays - wheat

2010-05-27 | E-GEOD-15864 | biostudies-arrayexpress

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2005-09-01 | E-MEXP-330 | biostudies-arrayexpress

Xenobiotic response in Drosophila melanogaster: sex dependence of P450 and GST gene induction

Project description:The effect of xenobiotics (phenobarbital and atrazine) on the expression of Drosophila melanogaster CYP genes encoding cytochromes P450, a gene family generally associated with detoxification, was analyzed by DNA microarray hybridization and verified by real time RT-PCR in adults of both sexes. Only a small subset of the 86 CYP genes was significantly induced by the xenobiotics. Eleven CYP genes and three GST genes were significantly induced by phenobarbital, seven CYP and one GST gene were induced by atrazine. Cyp6d5, Cyp6w1, Cyp12d1 and the ecdysone-inducible Cyp6a2 were induced by both chemicals. The constitutive expression of several of the inducible genes (Cyp6a2, Cyp6a8, Cyp6d5, Cyp12d1) was higher in males than in females, and the induced level similar in both sexes. Thus, the level of induction was consistently higher in females than in males. The female-specific and hormonally-regulated yolk protein genes were significantly induced by phenobarbital in males and repressed by atrazine in females. Our results suggest that the numerous CYP genes of Drosophila respond selectively to xenobiotics, providing the fly with an adaptive response to chemically adverse environments. The xenobiotic-inducibility of some CYP genes previously associated with insecticide resistance in laboratory-selected strains (Cyp6a2, Cyp6a8, Cyp12d1) suggests that deregulation of P450 gene expression may be a facile way to achieve resistance. Our study also suggests that xenobiotic-induced changes in P450 levels can affect insect fitness by interfering with hormonally-regulated networks. Keywords: induction by phenobarbital and atrazine in adults drosophila melanogaster of both sex.

2006-06-29 | GSE5125 | GEO

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