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Transcription profiling of human umbilical vein endothelial cells (HUVEC) treated with VEGF-A

ABSTRACT: Angiogenesis, the formation of new capillaries by sprouting from preexisting vessels, is mainly induced by VEGF-A. To identify genes which are induced by VEGF-A in endothelial cells, HUVEC were starved and induced by VEGF-A165 for 30, 60 and 150min. RNA of induced and uninduced cells was isolated and subjected to microarray analysis using Affymetrix microarray. Experiment Overall Design: Human umbilical vein endothelial cells were grown in complete EGM-2 medium in dense culture for four days without change of medium and before the stimulation in EGM-2 medium without growth factors over night. The cells were stimulated with 100ng/ml VEGF-A165 for 30, 60 and 150 min. RNA was isolated with Trizol according to the manufacturer's instructions.

ORGANISM(S): Homo sapiens

SUBMITTER: Julia Testori

PROVIDER: E-GEOD-15464 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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The VEGF-induced transcriptional response comprises gene clusters at the crossroad of angiogenesis and inflammation.

Schweighofer Bernhard B Testori Julia J Sturtzel Caterina C Sattler Susanne S Mayer Herbert H Wagner Oswald O Bilban Martin M Hofer Erhard E

Thrombosis and haemostasis 20090901 3

VEGF-A is the major trigger of vasculogenesis and physiologic angiogenesis. We have investigated to which extent the gene repertoire induced by VEGF-A in endothelial cells is distinct from that of other growth factors and inflammatory cytokines. Genes upregulated in human umbilical vein endothelial cells treated with VEGF, EGF or IL-1 were compared by microarray analysis and clusters characteristic for individual or combinations of inducers were defined. VEGF-A upregulated in comparison to EGF a ...[more]

PMID: 19718476

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