Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

ShRNA mediated knock-down of Tal1 in human Endothelial Colony Forming Cells (ECFCs)

ABSTRACT: Endothelial colony-forming cells (ECFCs) have been reported as promising cells for regenerative medicine thanks to their angiorepair properties. Transcription factors are primary determinants of the functional capacity of the cells and TAL1 has been shown as a critical regulator of endothelial lineage in both development and adult life. However, only few (three) TAL1 targets have been identified so far in mouse and human endothelial cells. This microarray experiment, where TAL1 expression was knocked-down, was designed to identify TAL1-dependent genes in primary human endothelial stem/progenitor cells. ECFCs were isolated from three independent cord blood samples (n=3, biological replicates) and cultured in complete EGM-2 medium. The knockdown of TAL1 was induced by infection with lentiviruses expressing an anti-TAL1 shRNA. A scrambled shRNA was used as a negative control. Cells were then harvested for RNA extraction. DNA-free total RNA was isolated with RNeasy Mini Kit and hybridized to the Affymetrix Human Gene 1.0 ST gene expression microarray.

ORGANISM(S): Homo sapiens

SUBMITTER: Alphonse Chu 

PROVIDER: E-GEOD-44444 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

Json Xml
altmetric image

Publications

A major goal of cell therapy for vascular diseases is to promote revascularization through the injection of endothelial stem/progenitor cells. The gene regulatory mechanisms that underlie endothelial progenitor-mediated vascular repair, however, remain elusive. Here, we identify the transcription factor TAL1/SCL as a key mediator of the vascular repair function of primary human endothelial colony-forming cells (ECFCs). Genome-wide analyses in ECFCs demonstrate that TAL1 activates a transcription  ...[more]

Similar Datasets

Genomics TAL1 in human Endothelial Colony-Forming Cells
2014-05-02 | E-GEOD-44546 | biostudies-arrayexpress
Genomics Comparative analysis of TAL1 binding in TSA-treated versus non-treated Endothelial Colony Forming Cells (ECFCs)
2014-05-06 | E-GEOD-53423 | biostudies-arrayexpress
Genomics Genomic binding profile of TAL1 in human Endothelial Colony-Forming Cells
2014-05-06 | E-GEOD-44442 | biostudies-arrayexpress
Transcriptomics PRD1-BF1 drives VEGF-mediated innate immune suppression in tumor vasculature
2015-07-10 | E-MTAB-2963 | biostudies-arrayexpress
Transcriptomics cDNA microarray expression data from human aortic smooth muscle cells compared for 9p21.3 coronary artery disease risk locus genotypes
2024-02-01 | E-MTAB-12709 | biostudies-arrayexpress
Transcriptomics Transcription profiling of human megakaryocytes treated with mild hyperthermia on Mk differentiation
2008-06-16 | E-GEOD-9218 | biostudies-arrayexpress
Transcriptomics Microarray expression profiling of male and female bone marrow derived macrophages from wild type and lysMCre/Irf2bp2flox mice
2024-09-01 | E-MTAB-14166 | biostudies-arrayexpress
Transcriptomics Human umbilical cord blood-derived endothelial colony forming cells under serum-free conditions
2010-07-29 | E-GEOD-20504 | biostudies-arrayexpress
Transcriptomics cDNA microarray expression data of heart, skeletal muscle and liver comparing Irf2bp2-null mice to wild type controls
2023-02-02 | E-MTAB-11558 | biostudies-arrayexpress
Transcriptomics Transcription profiling of human primitive bipotent and committed luminal progenitor cells as well as mature luminal and myoepithelial cells from normal mammary tissue
2008-06-20 | E-GEOD-11394 | biostudies-arrayexpress