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Acute Lymphocytic Leukemia versus associated xenografts

ABSTRACT: Leukemic cells from either peripheral blood or bone marrow were obtained from 6 patients with acute lymphocytic leukemia. These cells were used to generate mouse xenografts and a comparison was made between those two sample sources. Six pairs of human leukemia and associated xenografts are compared.

ORGANISM(S): Homo sapiens

SUBMITTER: Patrick Brown

PROVIDER: E-GEOD-15621 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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High frequencies of leukemia stem cells in poor-outcome childhood precursor-B acute lymphoblastic leukemias.

Morisot S S Wayne A S AS Bohana-Kashtan O O Kaplan I M IM Gocke C D CD Hildreth R R Stetler-Stevenson M M Walker R L RL Davis S S Meltzer P S PS Wheelan S J SJ Brown P P Jones R J RJ Shultz L D LD Civin C I CI

Leukemia 20100826 11

In order to develop a xenograft model to determine the efficacy of new therapies against primary human precursor-B acute lymphoblastic leukemia (ALL) stem cells (LSCs), we used the highly immunodeficient non-obese diabetic (NOD).Cg-Prkdc(scid)IL2rg(tmlWjl)/SzJ (NOD-severe combined immune deficient (scid) IL2rg(-/-)) mouse strain. Intravenous transplantation of 2 of 2 ALL cell lines and 9 of 14 primary ALL cases generated leukemia-like proliferations in recipient mice by 1-7 months after transpla ...[more]

PMID: 20739953

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