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Metabolism-based resistance to herbicides in black-grass

ABSTRACT: Resistance to herbicides in weeds can be due to alteration(s) in the gene encoding the herbicide target site, or to herbicide degradation via a deviation in plant general metabolism. If target-site-based resistance is easy to study, the multigenic control of metabolism-based resistance renders it much more complex to study. Metabolism-based resistance to herbicides represents the major part of herbicide resistance in black-grass. Its most likely basis is an overexpression of genes encoding enzymes degrading herbicides. We thus seek to identify such overexpressed genes by comparing the transcriptomes of resistant and sensitive black-grass individuals belonging to an F2 line segregating for two resistance genes. Given there are no genomic tools developed for black-grass, this approach will use heterologous hybridisation onto a wheat Affymetrix microarray. Comparison using heterologous hybridisation onto a wheat whole-genome microarray of transcriptome of three pools of black-grass plants obtained 2h30 after herbicide spraying at field rate. The three pools correspond to: Â· Sensitive plants (killed by herbicide). Â· Moderately resistant plants (growth impaired by herbicide but plants still alive) Â· Resistant plants (growth unimpaired by herbicide) 6 arrays - wheat

ORGANISM(S): Alopecurus myosuroides

SUBMITTER: Sandrine Balzergue

PROVIDER: E-GEOD-15864 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Metabolism-based resistance to herbicides in black-grass

Project description:Resistance to herbicides in weeds can be due to alteration(s) in the gene encoding the herbicide target site, or to herbicide degradation via a deviation in plant general metabolism. If target-site-based resistance is easy to study, the multigenic control of metabolism-based resistance renders it much more complex to study. Metabolism-based resistance to herbicides represents the major part of herbicide resistance in black-grass. Its most likely basis is an overexpression of genes encoding enzymes degrading herbicides. We thus seek to identify such overexpressed genes by comparing the transcriptomes of resistant and sensitive black-grass individuals belonging to an F2 line segregating for two resistance genes. Given there are no genomic tools developed for black-grass, this approach will use heterologous hybridisation onto a wheat Affymetrix microarray. Comparison using heterologous hybridisation onto a wheat whole-genome microarray of transcriptome of three pools of black-grass plants obtained 2h30 after herbicide spraying at field rate. The three pools correspond to: · Sensitive plants (killed by herbicide). · Moderately resistant plants (growth impaired by herbicide but plants still alive) · Resistant plants (growth unimpaired by herbicide)

2010-05-04 | GSE15864 | GEO

A key role for a glutathione transferase in multiple herbicide resistance in grass weeds

2013-03-13 | GSE42065 | GEO

A key role for a glutathione transferase in multiple herbicide resistance in grass weeds

Project description:The gene encoding a protein (AmGSTF1) associated with multiple herbicide resistance (MHR) in black-grass was transgenically expressed in Arabidopsis thaliana.The goal of this study was to determine if AmGSTF1 could elicit an MHR phenotype in the transgenic host. Affymetix microarrays were used to detect changes in transcriptional regulation between amgstf1-expressors and wild-type Arabidopsis plants (ecotype Col0). Total RNA was isolated from amgstf1-expressors and wild-type Arabidopsis plants (ecotype Col0) respectively, grown under identical growth room conditions. Performed in biological triplicate.

2013-03-13 | E-GEOD-42065 | biostudies-arrayexpress

Alopecurus myosuroides

Project description:Metabolism-based resistance to herbicides in black-grass

| PRJNA116967 | ENA

Transcriptome analysis indicates the involvement of herbicide-responsive and plant–pathogen interaction pathways in the development of resistance to ACCase inhibitors in Apera spica-venti

Project description:Background: The continuous use of the herbicides contributes to the emergence of the resistant populations of numerous weed species that are tolerant to multiple herbicides with different modes of action (multiple resistance) which is provided by non-target-site resistance mechanisms. In this study, we addressed the question of rapid acquisition of herbicide resistance to pinoxaden (acetyl CoA carboxylase inhibitor) in Apera spica-venti, which endangers winter cereal crops and has high adaptation capabilities to inhabit many rural locations. To this end, de novo transcriptome of Apera spica-venti was assembled and RNA-sequencing analysis of plants resistant and susceptible to pinoxaden treated with this herbicide was performed. Results: The obtained data showed that the prime candidate genes responsible for herbicide resistance were those encoding 3-ketoacyl-CoA synthase 12-like, UDP-glycosyltransferases (UGT) including UGT75K6, UGT75E2, UGT83A1-like, and glutathione S-transferases (GSTs) such as GSTU1 and GSTU6. Also, such highly accelerated herbicide resistance emergence may result from the enhanced constitutive expression of a wide range of genes involved in detoxification already before herbicide treatment and may also influence response to biotic stresses, which was assumed by the detection of expression changes in genes encoding defence-related proteins, including receptor kinase-like Xa21. Moreover, alterations in the expression of genes associated with methylation in non-treated herbicide-resistant populations were identified. Conclusion: The obtained results indicated genes that may be involved in herbicide resistance. Moreover, they provide valuable insight into the possible effect of resistance on the weed interaction with the other stresses by indicating pathways associated with both abiotic and biotic stresses.

2023-04-22 | GSE204857 | GEO

Transcriptome analysis indicates the involvement of herbicide-responsive and plant-pathogen interaction pathways in the development of resistance to ACCase inhibitors in Apera spica-venti

2023-04-22 | GSE204788 | GEO

Transcription profiling of field grown wheat treated with 3 herbicides

Project description:Wheat plants, grown in the field, were treated with 3 herbicides commonly applied on this crop to determine potential changes in gene expression.

2005-09-01 | E-MEXP-330 | biostudies-arrayexpress

Transcriptome response to safener treatment in rice cell cultures

Project description:Safeners are an important group of agrochemicals that can extend the selective use of existing herbicides into a wider range of cereals by protecting the crops from chemical injury. The recently released aromatic sulphonamide safeners cyprosulfamide and metcamifen were tested for their ability to protect rice plants from the phytotoxicity caused by clodinafop-propargyl, a herbicide normally only used in wheat. Transcriptome studies in rice cultures demonstrated that whereas cyprosulfamide had a negligible effect on gene expression over a 4 h exposure, metcamifen perturbed the abundance of 590 transcripts. Analysis of the inducible genes suggested that safening elicited similar gene families to those associated with specific biotic and abiotic stresses, notably those elicited by abscisic acid, salicylic acid and methyl-jasmonate.

2019-06-01 | GSE124666 | GEO

Transcription profiling of wheat treated with 3 herbicides grown in controlled conditions

Project description:Wheat plants grown in controlled conditions were treated with 3 herbicides commonly applied on this crop to determine the potential effects of these compounds on gene expression.

2005-09-01 | E-MEXP-329 | biostudies-arrayexpress

Expression profiling of grass-clump dwarf in synthetic hexaploids from triploid hybrids crossed between tetraploid wheat and Aegilops tauschii

Project description:We have employed whole genome microarray expression profiling as a discovery platform to identify genes to alter the transcript accumulation levels in grass-clump dwarf lines, which are synthetic hexaploid lines from triploid hybrids crossed between tetraploid wheat (Triticum turgidum ssp. durum cv. Langdon or T. turgidum ssp. carthlicum) and diploid wheat progenitor Aegilops tauschii (KU2025). No up-regulation of defense-related genes was observed under the normal temperature, and down-regulation of wheat APETALA1-like MADS-box genes, considered to act as flowering promoters, was found in the grass-clump dwarf lines. Together with small RNA sequencing analysis of the grass-clump dwarf line, unusual expression of the miR156/SPLs module could explain the grass-clump dwarf phenotype. Expression patterns were compared between the three synthetic hexaploid lines showing the wild-type phenotype (as a reference) and grass-clump dwarf. Total RNA samples were isolated from crown tissues of the plants grown at 24Â°C under long day (18-h light and 6-h dark) condition for 8 weeks. Two independent experiments were conducted in each exprement.

2016-03-02 | E-GEOD-78784 | biostudies-arrayexpress

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