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Transcription profiling of mouse murine dorsal neural tube bisected along the midline with one half from each embryo used for control and the other half treated with 10-6M RA dissolved in ethanol for 6, 12, 24 or 48 h


ABSTRACT: This series represents murine dorsal neural tube bisected along the midline with one half from each embryo used for control and the other half treated with 10-6M RA dissolved in ethanol for 6, 12, 24 or 48 h. For 6 h exposures, the explants were cultured overnight on fibronectin coated 35mm dishes (Biocoat, Becton Dickinson Labware, Bedford, MA) in DMEM with 10% horse serum in order to allow for sufficient outgrowth of neural crest cells. The RA was added the following morning; RMA Express 0.2 used to initially normalize data; GeneSpring (Silicon Genetics, Inc.) used for subsequent analysis; Samples were analyzed at 6, 12, 24, and 48 hours.

ORGANISM(S): Mus musculus

SUBMITTER: Sarah Williams 

PROVIDER: E-GEOD-1588 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Large-scale reprogramming of cranial neural crest gene expression by retinoic acid exposure.

Williams Sarah S SS   Mear John P JP   Liang Hung-Chi HC   Potter S Steven SS   Aronow Bruce J BJ   Colbert Melissa C MC  

Physiological genomics 20041001 2


Although retinoic acid (RA), the active form of vitamin A, is required for normal embryonic growth and development, it is also a powerful teratogen. Infants born to mothers exposed to retinoids during pregnancy have a 25-fold increased risk for malformations, nearly exclusively of cranial neural crest-derived tissues. To characterize neural crest cell responses to RA, we exposed murine crest cultures to teratogenic levels of RA and subjected their RNA to microarray-based gene expression profile  ...[more]

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