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Gene expression analysis in the adult carotid body stem cell niche

ABSTRACT: The carotid body (CB) is a peripheral chemoreceptor organ that suffers a profound angiogenic and neurogenic process in response to hypoxemia, to contribute to physiological adaptation of the organism. We have previously described a subpopulation of neural crest-derived stem cells residing in the organ. These cells are responsible for hypoxia-induced neurogenesis. Little is known about the molecular mechanisms underlying this neurogenic process. We have used neuronal cell-enriched neurosphere (NS) cultures vs. undifferentiated cell-enriched NS to study molecular pathways involved in the biology of these CB different cell types.

ORGANISM(S): Rattus norvegicus

SUBMITTER: Ricardo Pardal

PROVIDER: E-GEOD-67429 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Gene expression analysis in the adult carotid body stem cell niche

Project description:The carotid body (CB) is a peripheral chemoreceptor organ that suffers a profound angiogenic and neurogenic process in response to hypoxemia, to contribute to physiological adaptation of the organism. We have previously described a subpopulation of neural crest-derived stem cells residing in the organ. These cells are responsible for hypoxia-induced neurogenesis. Little is known about the molecular mechanisms underlying this neurogenic process.

2015-03-31 | GSE67429 | GEO

Expression data from carotid body glomus cells and neuroblasts, from young adult rats (9-11 weeks of age)

Project description:The carotid body is a peripheral chemoreceptor organ containing a neurogenic niche with stem cells and immature neuronal cells that convert into new neurons (glomus cells) upon activation by hypoxia. We have used microarray to compare the gene expression programs of glomus cells and neuroblasts, both in normoxic and hypoxic (3 days) conditions, to highlight the molecular mechanisms underlying fast neurogenesis

2023-12-17 | GSE249594 | GEO

Single cell transcriptome analysis of mouse carotid body glomus cells

Project description:The carotid body (CB) is a major arterial chemoreceptor containing glomus cells whose activities are regulated by changes in arterial blood contents including oxygen. We employed the single cell RNASeq method by performing next-generation sequencing on single glomus cellderived cDNAs to eliminate contamination of genes derived from other cell types present in the CB. This study has generated a CB expression profile containing novel glomus cell specific genes. The wealth of information provided through this study offers a valuable foundation for identifying molecules functioning in the CB.

2016-03-15 | GSE76579 | GEO

Gene expression analysis in NB adherent primary cell lines after endothelin-1 treatment

Project description:We cultured adherent primary cell lines from NB tumor samples. Adherent primary cell lines from NB tumor samples have a subpopulation of neural crest progenitors that grow as spheres when cultured in low-binding conditions. We tested the changes in gene expression induced by endothelin-1 (ET1), a cytokine that regulates proliferation, migration, differentiation and survival of NC cells .

2016-02-25 | E-GEOD-78268 | biostudies-arrayexpress

Single cell transcriptome analysis of mouse carotid body glomus cells

Project description:The carotid body (CB) is a major arterial chemoreceptor containing glomus cells whose activities are regulated by changes in arterial blood contents including oxygen. We employed the single cell RNAÂSeq method by performing next-generation sequencing on single glomus cellÂderived cDNAs to eliminate contamination of genes derived from other cell types present in the CB. This study has generated a CB expression profile containing novel glomus cell specific genes. The wealth of information provided through this study offers a valuable foundation for identifying molecules functioning in the CB. 8 single CB glomus cells that were confirmed expressing tyrosine hydroxylase (Th), ubiquitin carboxyl-terminal esterase L1 (Uchl1) and potassium channel subfamily K member 3 (Kcnk3) were harvested from P4-5 wild type mice and subjected to single-cell RNA-Seq

2016-03-15 | E-GEOD-76579 | biostudies-arrayexpress

Single cell RNA-seq analysis of feeder-free iPSC-derived gliogenic NS/PCs and neurogenic NS/PCs

Project description:We performed single cell RNA sequencing to analyze the transcriptional profile of gliogenic NS/PCs and neurogenic NS/PCs derived from the same parental feeder-free iPSCs.

2020-12-09 | GSE138302 | GEO

Comparative analysis of transcriptomes from the carotid body, adrenal medulla, and superior cervical ganglion of adult mice

Project description:Acute O2 sensing is a fundamental property of cells in the peripheral chemoreceptors, e.g. glomus cells in the carotid body (CB) and chromaffin cells in the adrenal medulla (AM), and is necessary for adaptation to hypoxia. The mechanisms underlying the detection of changes in O2 tension by these cells are still poorly understood. Recently, we suggested that CB glomus cells have specific metabolic features that favour their sensitivity to hypoxia (Fernandez-Aguera et al, 2015, Cell Metab.). To investigate the metabolic profile characteristic of acute O2-sensing cells, we used adult mice to compare the transcriptomes of three organs derived from common sympathoadrenal progenitors, but exhibiting variable responsiveness to acute hypoxia: CB and AM which are O2-sensitive (CB > AM) and superior cervical ganglion (SCG), which are practically O2-insensitive. Our data support the concept that responsiveness to acute hypoxia depends on a “signature metabolic profile” in peripheral chemoreceptor cells.

2017-09-08 | GSE99593 | GEO

Transcription profiling of mouse murine dorsal neural tube bisected along the midline with one half from each embryo used for control and the other half treated with 10-6M RA dissolved in ethanol for 6, 12, 24 or 48 h

Project description:This series represents murine dorsal neural tube bisected along the midline with one half from each embryo used for control and the other half treated with 10-6M RA dissolved in ethanol for 6, 12, 24 or 48 h. For 6 h exposures, the explants were cultured overnight on fibronectin coated 35mm dishes (Biocoat, Becton Dickinson Labware, Bedford, MA) in DMEM with 10% horse serum in order to allow for sufficient outgrowth of neural crest cells. The RA was added the following morning; RMA Express 0.2 used to initially normalize data; GeneSpring (Silicon Genetics, Inc.) used for subsequent analysis; Samples were analyzed at 6, 12, 24, and 48 hours.

2007-10-11 | E-GEOD-1588 | biostudies-arrayexpress

Transcription profiling of mouse HoxA2 knockout

Project description:Transcription profiling was performed of second branchial arches of E11.5 embryos from Hoxa2+/- intercrosses. After genotyping the embryos, wild type and Hoxa2-/- were profiled by microarray.

2011-12-01 | E-MEXP-3261 | biostudies-arrayexpress

Norepinephrine transporter-deficient neural crest cells

Project description:Background: The goal of this study was to determine the transcriptional consequences of norepinephrine transporter (NET) gene deletion in noradrenergic neuron differentiation. The norepinephrine transporter (NET) is the target of powerful mind-altering substances, such as tricyclic antidepressants and the drug of abuse, cocaine. NET function in adult noradrenergic neurons of the peripheral and central nervous systems is that of a scavenger that internalizes norepinephrine from the synaptic cleft. By contrast, norepinephrine (NE) transport has a different role in embryogenesis. It promotes differentiation of neural crest cells and locus ceruleus progenitors into noradrenergic neurons, whereas NET inhibitors, such as the tricyclic antidepressant desipramine and the drug of abuse, cocaine, inhibit noradrenergic differentiation. While NET structure und regulation of NET function is well described, little is known about downstream targets of NE transport. Results: We have determined by long serial analysis of gene expression (LongSAGE) the gene expression profiles of in vitro differentiating wild type and norepinephrine transporter-deficient (NETKO) neural crest derivatives. Comparison analyses with the wild type library (GSM 105765) have identified a number of important differentially expressed genes, including genes relevant to noradrenergic neuron differentiation and to the phenotype of NETKO mice. Furthermore we have identified novel differentially expressed genes. Conclusions: Loss of NET function during embryonic development deregulates signaling pathways that are critically involved in neural crest formation and noradrenergic neuron differentiation. The library was constructed from total RNA of 60 neural crest culture at culture day 7. The neural tubes were dissected from E9.5 embryos that lack the norepinephrine transporter gene.

2008-06-23 | E-GEOD-11788 | biostudies-arrayexpress

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