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Transcription profiling of rat insulinoma line INS-1 to identify targets of HNF1b mutants in INS-1

ABSTRACT: A rat insulinoma cell line (INS-1) was generated that contains a FRT site for FLP recombinase mediated site specific integration of specific genes. In addition, the line contains the tetracycline repressor allowing tetracycline (tet) induction of the introduced gene. By comparing the gene expression profiles of uninduced and tet-induced cells, target genes of introduced transcription factors HNF1b and HNF1b mutants were identified. Two different clones containing the same transcription factor were analyzed in the non-induced and tet-induced (24 h) state.

ORGANISM(S): Rattus norvegicus

SUBMITTER: Ludger Klein-Hitpass

PROVIDER: E-GEOD-1590 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Pattern of genes influenced by conditional expression of the transcription factors HNF6, HNF4alpha and HNF1beta in a pancreatic beta-cell line.

Thomas Heike H Senkel Sabine S Erdmann Silke S Arndt Tanja T Turan Gülüzar G Klein-Hitpass Ludger L Ryffel Gerhart U GU

Nucleic acids research 20041101 19

Using the rat insulinoma cell line INS-1 we generated beta-cell clones that are most efficient for gene transfer, as they contain an FRT site for Flp recombinase-mediated, site-directed integration of a single copy transgene. Therefore, the gene-of-interest can be introduced by DNA transfection without the need to select individual cell clones. Additionally, the clones contain the tetracycline repressor allowing tetracycline induction of the transgene. By oligonucleotide microarray we define the ...[more]

PMID: 15520459

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Targets of HNF1b mutants in INS-1

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