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Transcription profiling of rat insulinoma INS-1 cell lines (FLP-In T-REx)

ABSTRACT: Four rat insulinoma cell lines (INS-1) were generated that contain a FRT site for FLP recombinase mediated site specific integration of specific genes. In addition, the cell lines contain the tetracycline repressor allowing tetracycline (tet) induction of the introduced gene. By comparing the gene expression profiles of uninduced and tet-induced cells, target genes of introduced transcription factors or other signal tranduction molecules can be identified. The expression profiles of the parental INS-1 cell line and the four derivatives were determined on a RAE230A array, showing that the highly differentiated phenotype of the parental cell line was maintained in the clones.

ORGANISM(S): Rattus norvegicus

SUBMITTER: Ludger Klein-Hitpass 

PROVIDER: E-GEOD-1591 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Pattern of genes influenced by conditional expression of the transcription factors HNF6, HNF4alpha and HNF1beta in a pancreatic beta-cell line.

Thomas Heike H   Senkel Sabine S   Erdmann Silke S   Arndt Tanja T   Turan Gülüzar G   Klein-Hitpass Ludger L   Ryffel Gerhart U GU  

Nucleic acids research 20041101 19

Using the rat insulinoma cell line INS-1 we generated beta-cell clones that are most efficient for gene transfer, as they contain an FRT site for Flp recombinase-mediated, site-directed integration of a single copy transgene. Therefore, the gene-of-interest can be introduced by DNA transfection without the need to select individual cell clones. Additionally, the clones contain the tetracycline repressor allowing tetracycline induction of the transgene. By oligonucleotide microarray we define the  ...[more]

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