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Expression data from human colonic epithelial cells normal (N), adenoma (AD) or colorectal cancer (CRC) tissues

ABSTRACT: The whole-genome oligonucleotide microarray analysis of laser microdissected human colonic epithelial cells can contribute to determination of disease-specific expression alterations in colonic epithelial cells and to localize the origin the expression changes measured in whole biopsy samples. Keywords: whole genomic expression Total RNA was extracted from laser microdissected human colon epithelial cells and hybridized on Affymetrix HGU133 Plus 2.0 microarrays

ORGANISM(S): Homo sapiens

DISEASE(S): adenoma

SUBMITTER: Ferenc Sipos

PROVIDER: E-GEOD-15960 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Reversal of gene expression changes in the colorectal normal-adenoma pathway by NS398 selective COX2 inhibitor.

Galamb O O Spisák S S Sipos F F Tóth K K Solymosi N N Wichmann B B Krenács T T Valcz G G Tulassay Z Z Molnár B B

British journal of cancer 20100119 4

<h4>Background and aims</h4>Treatment of colorectal adenomas with selective cyclooxygenase-2 inhibitors can contribute to the chemoprevention of colorectal cancer (CRC), but the molecular background of their effect is not fully understood. We analysed the gene expression modulatory effect of N-(2-cyclohexyloxy-4-nitrophenyl)-methanesulfonamide (NS398) on HT29 cells to be correlated with expression data gained from biopsy samples.<h4>Methods</h4>HT29 colon adenocarcinoma cells were treated with N ...[more]

PMID: 20087348

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Expression data from human colonic epithelial cells normal (N), adenoma (AD) or colorectal cancer (CRC) tissues

Project description:The whole-genome oligonucleotide microarray analysis of laser microdissected human colonic epithelial cells can contribute to determination of disease-specific expression alterations in colonic epithelial cells and to localize the origin the expression changes measured in whole biopsy samples. Keywords: whole genomic expression

2010-03-03 | GSE15960 | GEO

Expression data from human colonic biopsy samples (adenoma-carcinoma)

Project description:Whole genomic microarray analysis was performed in order to identify gene expression profile alterations focusing on the dysplastic adenoma-carcinoma transition. Our aims were to determinate characteristic transcript sets for developing diagnostic mRNA expression patterns for objective classification of benign and malignant colorectal diseases and to test the classificatory power of these markers on an independent sample set. Total RNA was extracted from colonic biopsy samples of histologically negative patients and of patients with adenoma or colorectal cancer and were hybridized on Affymetrix HGU133 Plus 2.0 microarrays

2013-01-01 | E-GEOD-37364 | biostudies-arrayexpress

Expression data from human colonic biopsy sample

Project description:Gene expression profile based classification of colonic diseases are suitable for identification of diagnostic mRNA expression patterns which can establish the basis of a new molecular biological diagnostic method Keywords: whole genomic expression Total RNA was extracted from colonic biosy samples CRC and hybridized on Affymetrix HGU133 Plus 2.0 microarrays

2009-01-29 | E-GEOD-10714 | biostudies-arrayexpress

Gene expression profiles of components isolated from developing kidney at E11.5, E12.5 & E15.5 using LCM. (GUDMAP Series ID: 10)

Project description:The long term objective is to create an encyclopedia of the expression levels of all genes in multiple components of the developing kidney. The central thesis is straightforward. The combination of laser capture microdissection (LCM) plus microarray analysis offers a powerful, efficient and effective method for the creation of a global gene expression atlas of the developing kidney. Microarrays with essentially complete genome coverage can be used to quantitate expression levels of every gene in laser capture microdissected components of the developing kidney. The ensuing rapid read-out provides an expression atlas that is more sensitive, more economical and more complete than would be possible by in situ hybridizations alone. Keywords: Comparison of kidney components. At different developmental time points we isolate discrete elements of the kidney by using laser capture microdissection and then define their gene expression profiles with microarrays.

2006-11-18 | E-GEOD-6290 | biostudies-arrayexpress

Expression data from human peripheral blood samples

Project description:Gene expression profile based classification of colonic diseases are suitable for identification of diagnostic mRNA expression patterns which can establish the basis of a new molecular biological diagnostic method. Keywords: whole genomic expression Total RNA was extracted from peripheral blood samples Diverticulitis and Infective colitis were hybridized on Affymetrix HGU133 Plus 2.0 microarrays.

2011-03-01 | E-GEOD-21826 | biostudies-arrayexpress

Gene expression analysis of male and female embryonic day 14 genital tubercle (GUDMAP Series ID: 45).

Project description:The long term objective is to create an encyclopedia of the expression levels of all genes in multiple components of the developing urogenital system. The central thesis is straightforward. The combination of microdissected and laser capture microdissection (LCM) plus Microarray analysis offers a powerful, efficient and effective method for the creation of a global gene expression atlas of the developing urogenital system. Microarrays with essentially complete genome coverage can be used to quantitate expression levels of every gene. The ensuing rapid read-out provides an expression atlas that is more sensitive, more economical and more complete than would be possible by in situ hybridizations alone. The data submitted here represents the gene expression profiles of the embryonic day 14 genital tubercle of the male and the female. The genital tubercle region from E14 (TS22) FVB/N males and females were microdissected and total RNA isolated for gene expression analysis using the Affymetrix MOE430 microarray chip.

2012-12-04 | E-GEOD-42688 | biostudies-arrayexpress

Gene expression profiles of the P1 bladder isolated from SMAA-EYFP or SMAGA-EGFP (Acta2 or Actg2) transgenic and wild type mice. (GUDMAP Series ID: 2)

Project description:The long term objective is to create an encyclopedia of the expression levels of all genes in multiple components of the developing bladder. The central thesis is straightforward. The combination of laser capture microdissection (LCM) plus microarray analysis offers a powerful, efficient and effective method for the creation of a global gene expression atlas of the developing bladder. Microarrays with essentially complete genome coverage can be used to quantitate expression levels of every gene in laser capture microdissected components of the developing bladder. The ensuing rapid read-out provides an expression atlas that is more sensitive, more economical and more complete than would be possible by in situ hybridizations alone. As a first step we sought to determine if expression of the reporter gene (EGFP or EYFP) used to identify smooth muscle cells altered the gene expression profile of the bladder. Keywords: Comparison of postnatal day 1 bladders. Postnatal day 1 bladders were isolated from transgenic and wild-type mice to determine the effects of transgene expression on the gene expression profile in the bladder. Details of the GUDMAP project can be found at http://www.gudmap.org/index.html

2006-05-12 | E-GEOD-4816 | biostudies-arrayexpress

Gene expression profiles of E13 bladder compartments. (GUDMAP Series ID: 24)

Project description:The long term objective is to create an encyclopedia of the expression levels of all genes in multiple components of the developing bladder. The central thesis is straightforward. The combination of microdissected tissues and FACS sorted cells plus microarray analysis offers a powerful, efficient and effective method for the creation of a global gene expression atlas of the developing urogenital system. Microarrays with essentially complete genome coverage can be used to quantitate expression levels of every gene. The ensuing rapid read-out provides an expression atlas that is more sensitive, more economical and more complete than would be possible by in situ hybridizations alone. The data submitted here delineates the gene expression profiles of the mesenchymal and epithelial compartments of the e13 mouse bladder. Keywords: Mouse embryonic day 13 bladder mesenchyme and epithelium. FVB/N mice were time mated. At embryonic day 13 mice were euthanized by decapitation and the bladders were microdissected, cut just above the ureters, and treated with 20 µM EDTA for 20 minutes. Mesenchymal and epithelial compartments were then separated by rimming the bladder with a needle and harvested in RLT. Total RNA was isolated for gene expression analysis using the Affymetrix MOE430 microarray chip

2009-01-16 | E-GEOD-12618 | biostudies-arrayexpress

Expression data from human colonic biopsy samples

Project description:Age-related transcriptome changes can contribute to better understanding of gastrointestinal disorders such as colorectal cancer in older population Total RNA was extracted from colonic biopsy samples from histologically normal children and the samples were hybridized on Affymetrix HGU133 Plus 2.0 microarrays

2013-01-01 | E-GEOD-37267 | biostudies-arrayexpress

Gnotobiotic mouse cecal epithelium; B.thetaiotaomicron-B. longum mono- versus bi-association, PR chow, NMRI

Project description:Laser captured cecal epithelium from gnotobiotic mice colonized with B. thetaiotaomicron or B. longum (mono-association) or B. thetaiotaomicron bi-association with B. longum. Samples were derived from 10d colonizations of NMRI mice fed a standard-chow polysaccharide rich (PR) diet. Keywords: Germ-free vs. Mono-associations or Bi-association, in vivo Total RNA was prepared from laser captured cecal epithelium of 10 day associated gnotobiotic mice, double amplified, and hybridized to 430 2.0 GeneChips or to GlycoGeneChipsv1.

2010-10-08 | E-GEOD-5870 | biostudies-arrayexpress

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