Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Expression profiling of rainbow trout testicular development and spermatogenesis

ABSTRACT: Trout spermatogenesis proceeds seasonally in a way that all morphological and cellular events tend to be synchronized. We thus used gonads at key stages of the male reproductive cycle together with isolated germ cell populations to study the changes in gene expression underlying testis development. Statistical analysis followed by clustering of expression profiles allowed the discrimination of sequential events of gene activation or repression during testis development and/or throughout the germline. 38 samples covering 6 developmental stages (as defined in Gomez et al. 1998 Biol. Reprod. 58, 483-491) and 3 isolated germ cell populations were analysed. This included: - Testes at early stages containing slowly-dividing type A spermatogonia (Stage_I, n=5) or growing numbers of actively-dividing type B spermatogonia (Stages_IIa, n= 4; Stage_IIb, n= 4) - Maturing testes containing in addition large numbers of meiotic spermatocytes (Stage_IIIb, n=3) and post-meiotic spermatids (Stage_V, n=4) - Spawning testes containing essentially mature spermatozoa (Stage_VIII, n=3) - Fractions of isolated germ cells enriched in spermatogonia (Spermatogonia, N=6), spermatocytes (Spermatocyte, N=6) and spermatids (Spermatid, N=3).

ORGANISM(S): Oncorhynchus mykiss

SUBMITTER: Marie-JosÃ© Ricordel

PROVIDER: E-GEOD-16029 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Expression profiling of rainbow trout testis development identifies evolutionary conserved genes involved in spermatogenesis.

Rolland Antoine D AD Lareyre Jean-Jacques JJ Goupil Anne-Sophie AS Montfort Jérôme J Ricordel Marie-Jo MJ Esquerré Diane D Hugot Karine K Houlgatte Rémi R Chalmel Fréderic F Le Gac Florence F

BMC genomics 20091120

<h4>Background</h4>Spermatogenesis is a late developmental process that involves a coordinated expression program in germ cells and a permanent communication between the testicular somatic cells and the germ-line. Current knowledge regarding molecular factors driving male germ cell proliferation and differentiation in vertebrates is still limited and mainly based on existing data from rodents and human. Fish with a marked reproductive cycle and a germ cell development in synchronous cysts have p ...[more]

PMID: 19925684

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Dataset Information

Expression profiling of rainbow trout testicular development and spermatogenesis

Publications

Expression profiling of rainbow trout testis development identifies evolutionary conserved genes involved in spermatogenesis.

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