Trf4p in vivo crosslinking and ribonucleoprotein-immunopurification-chip analysis (X-RIP-Chip)
Ontology highlight
ABSTRACT:
ORGANISM(S): Saccharomyces cerevisiae
SUBMITTER: Andre Gerber
PROVIDER: E-GEOD-16105 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
Publications
Distinct roles of non-canonical poly(A) polymerases in RNA metabolism.
San Paolo Salvatore S Vanacova Stepanka S Schenk Luca L Scherrer Tanja T Blank Diana D Keller Walter W Gerber André P AP
PLoS genetics 20090710 7
Trf4p and Trf5p are non-canonical poly(A) polymerases and are part of the heteromeric protein complexes TRAMP4 and TRAMP5 that promote the degradation of aberrant and short-lived RNA substrates by interacting with the nuclear exosome. To assess the level of functional redundancy between the paralogous Trf4 and Trf5 proteins and to investigate the role of the Trf4-dependent polyadenylation in vivo, we used DNA microarrays to compare gene expression of the wild-type yeast strain of S. cerevisiae w ...[more]
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