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Transcription profiling of mouse total pancreatic RNA from 3 week old NOD.scid, NOD, BDC2.5/NOD and BDC2.5/NOD.scid animals

ABSTRACT: Total pancreatic RNA was isolated from 3 week old NOD.scid, NOD, BDC2.5/NOD and BDC2.5/NOD.scid animals by GITC method. Targets were produced using standard Affymetrix procedures from about 10ug total RNA. The data from NOD.scid, NOD, BDC2.5/NOD and BDC2.5/NOD.scid Affymetrix MGU74Av2 cel files was converted into Robust Multi Array (RMA) text file for analysis using GeneSpring 6.1

ORGANISM(S): Mus musculus

DISEASE(S): insulitis and diabetes

SUBMITTER: Jonathan Katz

PROVIDER: E-GEOD-1623 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Dynamic interaction between T cell-mediated beta-cell damage and beta-cell repair in the run up to autoimmune diabetes of the NOD mouse.

Vukkadapu Sankaranand S SS Belli Jenine M JM Ishii Koji K Jegga Anil G AG Hutton John J JJ Aronow Bruce J BJ Katz Jonathan D JD

Physiological genomics 20050125 2

In type 1 diabetes mellitus (T1DM), also known as autoimmune diabetes, the pathogenic destruction of the insulin-producing pancreatic beta-cells is under the control of and influenced by distinct subsets of T lymphocytes. To identify the critical genes expressed by autoimmune T cells, antigen presenting cells, and pancreatic beta-cells during the evolution of T1DM in the nonobese diabetic (NOD) mouse, and the genetically-altered NOD mouse (BDC/N), we used functional genomics. Microarray analysis ...[more]

PMID: 15671250

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