Unknown,Transcriptomics,Genomics,Proteomics

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IFN-alpha induced gene expression profile in NOD/BDC2.5 and NOD mice.

ABSTRACT: With the goal of identifying changes in gene expression in CD4(+) T cells during the development of diabetes in the nonobese diabetic (NOD) mouse, we used DNA microarrays to analyze gene expression in CD4(+) T cells from the pancreatic draining lymph nodes of NOD/BDC 2.5 T cell receptor transgenic and WT NOD mice at different ages. At 4 and 6 weeks of age, we found up-regulation of a number of genes that are known to be induced by IFN-alpha. IFN-alpha levels and IFN-alpha-producing plasmacytoid dendritic cells were increased in the PLNs of 3- to 4-week-old NOD mice. Moreover, blockade of IFN-alpha receptor 1 in NOD mice by a neutralizing antibody at 2-3 weeks of age significantly delayed the onset and decreased the incidence of type 1 diabetes, increased the relative number of immature dendritic cells in the PLNs, and enhanced the ability of spleen CD4(+) T cells to produce IL-4 and IL-10. These findings demonstrate that IFN-alpha in the PLNs is an essential initiator in the pathogenesis of type 1 diabetes in NOD mice. An all pairs experiment design type is where all labeled extracts are compared to every other labeled extract.

ORGANISM(S): Mus musculus

SUBMITTER: Qing Li

PROVIDER: E-GEOD-15213 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Interferon-alpha initiates type 1 diabetes in nonobese diabetic mice.

Li Qing Q Xu Baohui B Michie Sara A SA Rubins Kathleen H KH Schreriber Robert D RD McDevitt Hugh O HO

Proceedings of the National Academy of Sciences of the United States of America 20080820 34

With the goal of identifying changes in gene expression in CD4(+) T cells during the development of diabetes in the nonobese diabetic (NOD) mouse, we used DNA microarrays to analyze gene expression in CD4(+) T cells from the pancreatic draining lymph nodes of NOD/BDC 2.5 T cell receptor transgenic and WT NOD mice at different ages. At 4 and 6 weeks of age, we found up-regulation of a number of genes that are known to be induced by IFN-alpha. IFN-alpha levels and IFN-alpha-producing plasmacytoid ...[more]

PMID: 18716002

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Dataset Information

IFN-alpha induced gene expression profile in NOD/BDC2.5 and NOD mice.

Publications

Interferon-alpha initiates type 1 diabetes in nonobese diabetic mice.

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