Unknown,Transcriptomics,Genomics,Proteomics

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Gene expression in a Highly Tumorigenic Cells (HTC) purified from Urothelial Cancer

ABSTRACT: We used DNA microarrays to compare RNA transcript expression from distinct cell populations sorted by FACS with an antibody against the 67Kd laminin receptor (67LR), and showing distinct tumorigenicity in vivo. Sorted cells were derived by digesting xenografted sw 780 tumors into single cell suspensions. Differential gene expression was assessed by direct comparisons of labeled moieties, using a dye-swap design and biological replication. Hybridizations were performed on Agilent (Santa Clara, CA) Whole Human Genome DNA microarrays (hgug4112a). Three different samples were analyzed: 1) Urothelial cancer cells expressing 67LR (67LR+, BRIGHT); 2) Urothelial cancer cells not expressing 67LR (67LR-, DIM); 3) Control sorted urothelial cancer cells (BULK, containing both BRIGHT and DIM cells). In this series we provide normalized single channel intensities, aligned with the corresponding raw expression data.

ORGANISM(S): Homo sapiens

SUBMITTER: Luigi Marchionni 

PROVIDER: E-GEOD-16255 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Differentiation of a highly tumorigenic basal cell compartment in urothelial carcinoma.

He Xiaobing X   Marchionni Luigi L   Hansel Donna E DE   Yu Wayne W   Sood Akshay A   Yang Jie J   Parmigiani Giovanni G   Matsui William W   Berman David M DM  

Stem cells (Dayton, Ohio) 20090701 7

Highly tumorigenic cancer cell (HTC) populations have been identified for a variety of solid tumors and assigned stem cell properties. Strategies for identifying HTCs in solid tumors have been primarily empirical rather than rational, particularly in epithelial tumors, which are responsible for 80% of cancer deaths. We report evidence for a spatially restricted bladder epithelial (urothelial) differentiation program in primary urothelial cancers (UCs) and in UC xenografts. We identified a highly  ...[more]

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