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Transcription profiling by array of human renal proximal tubular cells of young or senescent replicative age; or high or low reactive oxygen species.

ABSTRACT: Renal Proximal Tubular Cells of young / senescent replicative age or high / low reactive oxygen species content were profiled using an Affymetrix Chip Platform.
Sample classifications:
Young refers to the in vitro age of early passage cells.
Senescent indicates cells at the end of their in vitro life span, having entered an irreversible growth arrest.
SortHI refers to cells with high intracellular reactive oxygen species content that were sorted by FACS according to a bright FL1 fluorescence after staining with 1 ï¾µM CM-H2DCFDA (Molecular Probes Cat No C6827).
SortLO refers to cells with low intracellular reactive oxygen species content that were sorted by FACS according to a dim FL1 fluorescence after staining with 1 ï¾µM CM-H2DCFDA.

ORGANISM(S): Homo sapiens

SUBMITTER: Matthias Hackl

PROVIDER: E-MEXP-2683 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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