Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

59 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

14028 versus che mutants

ABSTRACT: Salmonella wild type strain 14028 compared with chemotaxis mutants, cheA, cheB, cheY, and cheZ at various time points both in broth medium and on swarming plates

ORGANISM(S): Salmonella enterica subsp. enterica serovar Typhimurium

SUBMITTER: Michael McClelland

PROVIDER: E-GEOD-1632 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Publications

Sensing wetness: a new role for the bacterial flagellum.

Wang Qingfeng Q Suzuki Asaka A Mariconda Susana S Porwollik Steffen S Harshey Rasika M RM

The EMBO journal 20050505 11

We have uncovered a new role for the bacterial flagellum in sensing external wetness. An investigation into why mutants in the chemotaxis signaling pathway of Salmonella typhimurium exhibit fewer and shorter flagella than wild-type when propagated on a surface, first showed that the mutants downregulate only a small set of genes on swarm media--class 3 or 'late' motility genes, and genes associated with the pathogenicity island SPI-1 TTSS (type three secretion system). Based on observations that ...[more]

PMID: 15889148

Similar Datasets

14028 time course on swarming plates

Project description:Salmonella 14028 time course on swarming agar (0.6%) plates mRNA from 3 h time point on swarming plates used as reference for all the time course arrays

2010-10-08 | E-GEOD-1625 | biostudies-arrayexpress

Transcription profiles of che and flgM mutants of Typhimurium 14028 at 3h on agar plates

Project description:Transcription profiles of Typhimurium 14028 wt versus 14028 cheA, cheB, cheZ, flgM and flgM/cheY mutants at 3h on agar plates

2010-06-09 | E-GEOD-2341 | biostudies-arrayexpress

14028 cheY mutant time course expression on plate

Project description:time course of transcriptional profile of Typhimurium 14028 wt versus 14028 cheY mutant on agar plate

2010-06-09 | E-GEOD-2340 | biostudies-arrayexpress

WT 14028 versus mutant

Project description:WT 14028 versus mutant

2010-06-10 | E-GEOD-2455 | biostudies-arrayexpress

growth culture comparisons

Project description:All the other array data are here, including plate culture versus broth culture, swarming agar culture versus hard agar culture, wildtype versus mutants other than che, and so on

2010-10-08 | E-GEOD-1633 | biostudies-arrayexpress

14028 time course on non-swarming plates

Project description:Salmonella strain 14028, time course, on non-swarming agar plates (1.5%), array were done between reference mRNA (from swarmer cells at 3 hour) and mRNA from various time points on non-swarming plates.

2010-06-06 | E-GEOD-1631 | biostudies-arrayexpress

14028 time course in broth medium

Project description:Salmonella strain 14028, time course in broth medium, array were done between mRNA from various time point in broth and reference mRNA, which is from swarmer cells at 3 hour on swarming plates Keywords: other

2004-09-02 | E-GEOD-1630 | biostudies-arrayexpress

Transcriptome of dehydrated vs hydrated Salmonella cells

Project description:Transcriptional profiles of dehydrated Salmonella Typhimurium SL1344 cells post 22h air drying were compared to those of SL1344 cells incubated for 22 h in DDW Two-condition experiment, dehydrated versus hydrated (control) Salmonella cells. Biological replicates: 4 control, 4 dehydrated, independently grown and harvested. One replicate per array.

2012-12-03 | E-GEOD-38475 | biostudies-arrayexpress

Salmonella typhimurium igaA(T191P) and related

Project description:We have exploited a spontaneously isolated mutant IgaA(T191P) that is near-maximally activated for the Rcs system, to identify a vast set of genes that respond, and report new regulatory properties of this signaling system in Salmonella enterica serovar Typhimurium. Microarray data show that the Rcs system normally functions as a positive regulator of SPI-2 and other genes important for growth of Salmonella in macrophages, although when highly activated, the system completely represses the SPI-1/SPI-2 virulence, flagellar, and fimbrial biogenesis pathways. The auxilliary protein RcsA, which works with RcsB to positively regulate colanic acid and other target genes, not only stimulates but also antagonizes the positive regulation of many genes in the igaA mutant. We show that RcsB represses motility through the 'RcsB box' in the promoter region of the master operon flhDC, and that RcsA is not required for this regulation. Curiously, RcsB selectively stimulates expression of the flagellar Type 3 secretion genes fliPQR; an RcsAB box located downstream of fliR influences this regulation. We show that excess colanic acid impairs swimming and inhibits swarming motility, consistent with the inverse regulation of the two pathways by the Rcs system. The work was published in Journal of Bacteriology: Wang Q, Zhao Y, McClelland M, Harshey RM. The RcsCDB signaling system and swarming motility in Salmonella enterica serovar Typhimurium: dual regulation of flagellar and SPI-2 virulence genes. J Bacteriol. 2007 Sep 28; [Epub ahead of print] PMID: 17905992 [PubMed - as supplied by publisher] Keywords: Comparative genomic hybridization Microarray was used to reveal the gene expression profiles in igaA(T191P) and related strains at two growth stages (log and stationary phases). Quantitative RT-PCR was used for detail studies.

2008-06-24 | E-GEOD-9584 | biostudies-arrayexpress

Transcriptomic alterations induced by AT-EAE in mouse spinal cord

Project description:We compared RNA samples extracted from spinal cords of control (C) and AT-EAE (E) mice using the "Multiple Yellow" strategy. 4 distinct C-extracts were hybridized with two slides and 4 distinct E-extracts with other two slides, and the green/red normalized signals were compared separately and the E/C ratios averaged. Keywords = white matter Keywords = inflammation Keywords = cDNA microarray Keywords = experimental autoimmune encephalomyelitis

2010-06-10 | E-GEOD-2446 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data