Uncovering early response of gene regulatory networks in ES cells by systematic induction of transcription factors
Ontology highlight
ABSTRACT:
ORGANISM(S): Mus musculus
SUBMITTER: Minoru Ko
PROVIDER: E-GEOD-16375 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
Publications
Uncovering early response of gene regulatory networks in ESCs by systematic induction of transcription factors.
Nishiyama Akira A Xin Li L Sharov Alexei A AA Thomas Marshall M Mowrer Gregory G Meyers Emily E Piao Yulan Y Mehta Samir S Yee Sarah S Nakatake Yuhki Y Stagg Carole C Sharova Lioudmila L Correa-Cerro Lina S LS Bassey Uwem U Hoang Hien H Kim Eugene E Tapnio Richard R Qian Yong Y Dudekula Dawood D Zalzman Michal M Li Manxiang M Falco Geppino G Yang Hsih-Te HT Lee Sung-Lim SL Monti Manuela M Stanghellini Ilaria I Islam Md Nurul MN Nagaraja Ramaiah R Goldberg Ilya I Wang Weidong W Longo Dan L DL Schlessinger David D Ko Minoru S H MS
Cell stem cell 20091001 4
To examine transcription factor (TF) network(s), we created mouse ESC lines, in each of which 1 of 50 TFs tagged with a FLAG moiety is inserted into a ubiquitously controllable tetracycline-repressible locus. Of the 50 TFs, Cdx2 provoked the most extensive transcriptome perturbation in ESCs, followed by Esx1, Sox9, Tcf3, Klf4, and Gata3. ChIP-Seq revealed that CDX2 binds to promoters of upregulated target genes. By contrast, genes downregulated by CDX2 did not show CDX2 binding but were enriched ...[more]
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