Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of normal lung from 18 multi-organ donors to analyse variance of gene expression in lung

ABSTRACT: Peripheral sections of upper and lower lung from 18 human multi-organ donors without evidence of lung disease.

ORGANISM(S): Homo sapiens

SUBMITTER: Christopher Coldren 

PROVIDER: E-GEOD-1643 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Human lung project: evaluating variance of gene expression in the human lung.

Gruber Michael P MP   Coldren Christopher D CD   Woolum Malcolm D MD   Cosgrove Gregory P GP   Zeng Chan C   Barón Anna E AE   Moore Mark D MD   Cool Carlyne D CD   Worthen G Scott GS   Brown Kevin K KK   Geraci Mark W MW  

American journal of respiratory cell and molecular biology 20060223 1

Nondiseased tissue is an important reference for microarray studies of pulmonary disease. We obtained 23 single lungs from multiorgan donors at time of procurement. Donors varied in age, sex, smoking history, and ethnicity. Lungs were dissected into upper and lower lobe peripheral sections for RNA extraction. Microarray analysis was performed using Affymetrix Hu-133 Plus 2.0 arrays. We observed that the relative variability of gene expression increased rapidly from technical (lowest), to regiona  ...[more]

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