Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of rat immortalized SCN cells - circadian experiment


ABSTRACT: To determine whether immortalized cells derived from the rat SCN (SCN 2.2) retain intrinsic rhythm-generating properties characteristic of the SCN, oscillatory properties of the SCN2.2 transcriptome were analyzed and compared to those found in the rat SCN in vivo using rat U34A Affymetrix GeneChips. SCN2.2 cells were expanded in 6-well plates. At 6-hour interval across 2 circadian cycles, cells from single 6-well plates were harvested and pooled for total RNA extraction.

ORGANISM(S): Rattus norvegicus

SUBMITTER: Gus John Menger 

PROVIDER: E-GEOD-1654 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Circadian profiling of the transcriptome in immortalized rat SCN cells.

Menger Gus J GJ   Lu Kim K   Thomas Terry T   Cassone Vincent M VM   Earnest David J DJ  

Physiological genomics 20050315 3


Endogenous oscillations in gene expression are a prevalent feature of the circadian clock in the mammalian suprachiasmatic nucleus (SCN) and similar timekeeping systems in other organisms. To determine whether immortalized cells derived from the rat SCN (SCN2.2) retain these intrinsic rhythm-generating properties, oscillatory behavior of the SCN2.2 transcriptome was analyzed and compared with that found in the rat SCN in vivo using rat U34A Affymetrix GeneChips. In SCN2.2 cells, 116 unique genes  ...[more]

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