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Human Erythroid Day 10 ChIP using GATA, PolII, H3 and H4

ABSTRACT: A large-scale analysis of histone acetylation as well as RNA polymerase II and GATA 1 interactions on chromosome 6q in human erythroid progenitor cells. Human erythroid progenitors from day 10 erythroid cultures were used for ChIP-chip analysis. For this purpose, the NimbleGen array system was used, following manufacturer's protocols. Samples for ChIP were taken from the same individual and processed for use with each of the following antibodies: GATA1, PolII, AcH3 and AcH4.

ORGANISM(S): Homo sapiens

SUBMITTER: Jie Jiang

PROVIDER: E-GEOD-16541 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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The HBS1L-MYB intergenic interval associated with elevated HbF levels shows characteristics of a distal regulatory region in erythroid cells.

Wahlberg Karin K Jiang Jie J Rooks Helen H Jawaid Kiran K Matsuda Fumihiko F Yamaguchi Masao M Lathrop Mark M Thein Swee Lay SL Best Steve S

Blood 20090615 6

HBS1L-MYB intergenic polymorphism (HMIP) on chromosome 6q23 is associated with elevated fetal hemoglobin levels and has pleiotropic effects on several hematologic parameters. To investigate potential regulatory activity in the region, we have measured sensitivity of the sequences to DNase I cleavage that identified 3 tissue-specific DNase I hypersensitive sites in the core intergenic interval. Chromatin immunoprecipitation with microarray (ChIP-chip) analysis showed strong histone acetylation in ...[more]

PMID: 19528534

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