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Transcription profiling of human K562 cells overexpressing miR-34a

ABSTRACT: miR-34a is strongly induced upon TPA-induced megakaryocyte differentiation of K562 cells. To investigate the gene networks regulated by this miRNA during the process of differentiation we performed gene microarray analysis in K562 cells overexpressing miR-34a or a control sequence. Experiment Overall Design: K562 cells were transfected by nucleofection (Amaxa) with a miR-34a mimic (Dharmacon) or a control sequence with no homology to human genes. 24 hours post-transfection total RNA was extracted using trizol and hybridized on Affimetrix microarrays for comparing gene expression.

ORGANISM(S): Homo sapiens

SUBMITTER: Francisco Navarro

PROVIDER: E-GEOD-16674 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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miR-34a contributes to megakaryocytic differentiation of K562 cells independently of p53.

Navarro Francisco F Gutman David D Meire Eti E Cáceres Mario M Rigoutsos Isidore I Bentwich Zvi Z Lieberman Judy J

Blood 20090707 10

The role of miRNAs in regulating megakaryocyte differentiation was examined using bipotent K562 human leukemia cells. miR-34a is strongly up-regulated during phorbol ester-induced megakaryocyte differentiation, but not during hemin-induced erythrocyte differentiation. Enforced expression of miR-34a in K562 cells inhibits cell proliferation, induces cell-cycle arrest in G(1) phase, and promotes megakaryocyte differentiation as measured by CD41 induction. miR-34a expression is also up-regulated du ...[more]

PMID: 19584398

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