Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

NCI-H1299 lung cancer cell line transfected with miR-34a and ghR-34a


ABSTRACT: Analysis NCI-H1299 lung cancer cells transfected with synthetic oligo mimics for microRNAs (miRNAs) miR-34a and ghR-34a. We developed a 30-nucleotide single-strand RNA (ssRNA), termed “guide hairpin RNA (ghR),” that has a physiological function similar to that of miRNA and siRNA. The ghR caused no innate cytokine response either in vitro or in vivo. In addition, its structure does not contain a passenger strand seed sequence, reducing the potential for off-target effects relative to existing short RNA reagents. Systemic injection of ghR-form miR-34a (ghR-34a) suppressed tumor growth in a mouse model of RAS-induced lung cancer. Furthermore, ghR-34a functioned in a Dicer- and Ago2-independent manner. This novel RNAi technology may provide a novel, safe, and effective nucleic acid drug platform that will increase the clinical usefulness of nucleic acid therapy. MiR-34a–targeted mRNAs regulated by mRNA degradation rather than translational inhibition were identified using microarray data from miR-34 and ghR-34a transfectants.

ORGANISM(S): Homo sapiens

SUBMITTER: Shin-ichiro Ohno 

PROVIDER: E-GEOD-71423 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

Similar Datasets

2015-07-29 | GSE71423 | GEO
2012-03-26 | E-GEOD-34242 | biostudies-arrayexpress
2009-07-19 | E-GEOD-16674 | biostudies-arrayexpress
2012-08-08 | E-GEOD-38341 | biostudies-arrayexpress
2015-05-25 | E-GEOD-68740 | biostudies-arrayexpress
2019-10-14 | PXD015278 | Pride
2009-08-01 | E-MEXP-2241 | biostudies-arrayexpress
2008-06-15 | E-GEOD-7754 | biostudies-arrayexpress
2007-06-07 | GSE7754 | GEO
2021-02-01 | GSE161321 | GEO