Unknown,Transcriptomics,Genomics,Proteomics

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Comparative gene expression analysis of 2 subpopulations of dermal papilla cells.


ABSTRACT: Different types of hair follicles can be found in the skin of mice. It is believed that the signals that control hair follicle differentiation arise from cells in a structure called the dermal papilla. Understanding the nature of those signals is of interest for the biology of the normal tissue. We have developed a technique for isolation of dermal cells by enzymatic digestion of intact skin. We have identified two subpopulations of cells that can be separated by FACS. The Sox2-positive CD133-positive cells are found exclusively in the dermal papillae of guard/awl/auchene hairs, while Sox2-negative, CD133-positive cells are found in the other hair follicle types. We compared these populations with unfractionated dermal cells. We isolated the following 3 populations of cells from the back skin of neonatal mice (P2) by Flow Cytometry: 1) GFP-CD133- Total dermal cells 2) GFP-CD133+ Dermal Papilla cells 3) GFP+CD133+ Dermal Papilla cells The yield is approximately 50,000 cells of each population.

ORGANISM(S): Mus musculus

SUBMITTER: Ryan Driskell 

PROVIDER: E-GEOD-16801 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Sox2-positive dermal papilla cells specify hair follicle type in mammalian epidermis.

Driskell Ryan R RR   Giangreco Adam A   Jensen Kim B KB   Mulder Klaas W KW   Watt Fiona M FM  

Development (Cambridge, England) 20090715 16


The dermal papilla comprises the specialised mesenchymal cells at the base of the hair follicle. Communication between dermal papilla cells and the overlying epithelium is essential for differentiation of the hair follicle lineages. We report that Sox2 is expressed in all dermal papillae at E16.5, but from E18.5 onwards expression is confined to a subset of dermal papillae. In postnatal skin, Sox2 is only expressed in the dermal papillae of guard/awl/auchene follicles, whereas CD133 is expressed  ...[more]

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