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Comparability and concordance of replicated microarray data.

ABSTRACT: To measures the comparability and concordance of Illumina microarray, a series of 30 samples of Universal Human Reference RNA (UHRR) were set as controls for every single chip of total 30 Human-Ref V2 BeadChips. The average bead number of the 30 arrays was 42.3±8.1 for any bead type over the 22,184 probes. A high average correlation coefficient (r) value was obtained as 0.9908±0077 relative to each other of the expression intensity values from the 30 duplicate UHRR samples. 30 UHRR (Stratagene; La Jolla, CA) samples were used as controls for each of 30 BeadChips.

ORGANISM(S): Homo sapiens

SUBMITTER: Dong-Yu Wang

PROVIDER: E-GEOD-16984 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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A new gene expression signature, the ClinicoMolecular Triad Classification, may improve prediction and prognostication of breast cancer at the time of diagnosis.

Wang Dong-Yu DY Done Susan J SJ McCready David R DR Boerner Scott S Kulkarni Supriya S Leong Wey Liang WL

Breast cancer research : BCR 20110922 5

<h4>Introduction</h4>When making treatment decisions, oncologists often stratify breast cancer (BC) into a low-risk group (low-grade estrogen receptor-positive (ER+)), an intermediate-risk group (high-grade ER+) and a high-risk group that includes Her2+ and triple-negative (TN) tumors (ER-/PR-/Her2-). None of the currently available gene signatures correlates to this clinical classification. In this study, we aimed to develop a test that is practical for oncologists and offers both molecular cha ...[more]

PMID: 21939527

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Comparability and concordance of replicated microarray data.

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