Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of human acute myelogenous leukemia stem cells reveals disregulated gene networks


ABSTRACT: We performed the first genome-wide expression analysis directly comparing the expression profile of highly enriched normal human hematopoietic stem cells (HSC) and leukemic stem cells (LSC) from patients with acute myeloid leukemia (AML). Comparing the expression signature of normal HSC to that of LSC, we identified 3,005 differentially expressed genes. Using 2 independent analyses, we identified multiple pathways that are aberrantly regulated in leukemic stem cells compared with normal HSC. Several pathways, including Wnt signaling, MAP Kinase signaling, and Adherens Junction, are well known for their role in cancer development and stem cell biology. Other pathways have not been previously implicated in the regulation of cancer stem cell functions, including Ribosome and T Cell Receptor Signaling pathway. This study demonstrates that combining global gene expression analysis with detailed annotated pathway resources applied to highly enriched normal and malignant stem cell populations, can yield an understanding of the critical pathways regulating cancer stem cells. Experiment Overall Design: Total RNAs were isolated from both AML stem cells (9 patients) and normal bone marrow hematopoietic stem cells (HSCs) (4 control subjects).

ORGANISM(S): Homo sapiens

SUBMITTER: Qiang Tian 

PROVIDER: E-GEOD-17054 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Dysregulated gene expression networks in human acute myelogenous leukemia stem cells.

Majeti Ravindra R   Becker Michael W MW   Tian Qiang Q   Lee Tsung-Lu Michael TL   Yan Xiaowei X   Liu Rui R   Chiang Jung-Hsien JH   Hood Leroy L   Clarke Michael F MF   Weissman Irving L IL  

Proceedings of the National Academy of Sciences of the United States of America 20090213 9


We performed the first genome-wide expression analysis directly comparing the expression profile of highly enriched normal human hematopoietic stem cells (HSC) and leukemic stem cells (LSC) from patients with acute myeloid leukemia (AML). Comparing the expression signature of normal HSC to that of LSC, we identified 3,005 differentially expressed genes. Using 2 independent analyses, we identified multiple pathways that are aberrantly regulated in leukemic stem cells compared with normal HSC. Sev  ...[more]

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