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Expression profiling of sox11-expressing glioma-initiating cell-like cells

ABSTRACT: To identify factors involved in tumorigenicity of glioma-initiating cells (GICs), we compared gene expression in GIC-like cells with and without sox11 expression. We established sox11-expressing mouse glioma-initiating cell (GIC)-like cell line (NSCL61s), NSCL61s-sox11, which lost tumorigenicity when transplanted in vivo. We think that genes, which are differently expressed between NSCL61s and NSCL61s-sox11, would be new targets for glioma therapy.

ORGANISM(S): Mus musculus

SUBMITTER: Toru Kondo

PROVIDER: E-GEOD-17076 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Sox11 prevents tumorigenesis of glioma-initiating cells by inducing neuronal differentiation.

Hide Takuichiro T Takezaki Tatsuya T Nakatani Yuka Y Nakamura Hideo H Kuratsu Jun-ichi J Kondo Toru T

Cancer research 20091006 20

Recent findings have shown that malignant tumors contain cancer-initiating cells (CIC), which self-renew and are tumorigenic. However, CICs have not been characterized properly due to lack of specific markers. We recently established a mouse glioma cell line, NSCL61, by overexpressing an oncogenic HRas(L61) in p53-deficient neural stem cells. Using limiting dilution assays, we show that only 2 of 24 NSCL61 clones retained their tumorigenicity in vivo, although the others also expressed oncogenic ...[more]

PMID: 19808959

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Expression profiling of sox11-expressing glioma-initiating cell-like cells

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2009-12-01 | GSE17076 | GEO

Expression profiling of mouse glioma-initiating cell-like cells (GICs) and non-GICs

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Expression profiles of mouse glioma-initiating cells.

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Expression profiling of glioma initiating cells (GICs) in the sphere and differentiation conditions

Project description:Glioma initiating cells (GICs) are considered responsible for the therapeutic resistance and recurrence of malignant glioma. To clarify the molecular mechanism of GIC maintenance/differentiation, we established GIC clones from GBM patient tumors having the potential to differentiate into malignant gliomas in mouse intracranial xenograft, and established an in vitro glioma induction system by using serum stimulation. Upon the serum stimulation, the GIC spheres showed increased cellular proliferation, motility, filopodia/lameripodia formation and adhesion to the culture dishes. Simultaneously, the NSC marker proteins such as CD133 and Sox2 were down-regulated, and the astrocyte/glioma marker GFAP and the malignancy marker CD44 dramatically up-regulated. To identify genes/proteins whose expression changes dynamically during the differentiation of GICs into glioma cells, these GICs were subjected to DNA microarray/iTRAQ based integrated proteomics. Within 4 hours of tumor removal from GBM patients, tissues were subjected to GIC preparation. After successive cloning, total RNA from GIC clones (GIC03A and GIC03U) on day 2 or 7 of subculture in NSC medium with or without 10% FCS was subjected to the analysis with Affymetrix microarrays. Simultaneously, the proteins extracted from the same set of cells were subjected to LC-shot gun analyses using the 8-plex iTRAQ method. We sought to obtain the information of the common molecules that were up- or down-regulated during the GSC differentiation process, and functional targets for the early onset of GIC-associated glioma.

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Development of microRNA (miRNA) expression signatures for mouse glioma-initiating cells (GICs)

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Expression data from glioma cells exposed to interferon (IFN)-beta

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