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Unknown,Transcriptomics,Genomics,Proteomics

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Expression profiling of mouse glioma-initiating cell-like cells (GICs) and non-GICs

ABSTRACT: To identify factors involved in glioma-initiating cells (GICs), we compared gene expression between GIC-like cells and non-GICs. Neural stem cells (NSCs) were transfected with pCMS-EGFP-HRasL61 and pBabe-neo by electroporation and cultured in 0.5 mg/ml neomycin. The GFP-positive stable NSCs (NSCL61s) were purified by flow cytometry. Total RNA was prepared using RNeasy Mini Kit (QIAGEN).

ORGANISM(S): Mus musculus

SUBMITTER: Toru Kondo

PROVIDER: E-GEOD-17062 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Sox11 prevents tumorigenesis of glioma-initiating cells by inducing neuronal differentiation.

Hide Takuichiro T Takezaki Tatsuya T Nakatani Yuka Y Nakamura Hideo H Kuratsu Jun-ichi J Kondo Toru T

Cancer research 20091006 20

Recent findings have shown that malignant tumors contain cancer-initiating cells (CIC), which self-renew and are tumorigenic. However, CICs have not been characterized properly due to lack of specific markers. We recently established a mouse glioma cell line, NSCL61, by overexpressing an oncogenic HRas(L61) in p53-deficient neural stem cells. Using limiting dilution assays, we show that only 2 of 24 NSCL61 clones retained their tumorigenicity in vivo, although the others also expressed oncogenic ...[more]

PMID: 19808959

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Expression profiles of mouse glioma-initiating cells.

Project description:To identify factors involved in glioma-initiating cells (GICs), we compared gene expressions between GIC-like cells and non-GICs. p53-deficient Neural stem cells (NSCs), oligodendrocyte precursor cells (OPCs), and astrocytes (ASTs) were transfected with pCMS-EGFP-HRasL61 and pBabe-neo by electroporation and cultured in 0.5 mg/ml neomycin. The GFP-positive stable NSCs (NSCL61s), OPCs (OPCL61s) and ASTs (ASTL61s) were purified by flow cytometry. Total RNA was prepared using a RNeasy Mini Kit (QIAGEN) and used for the expression analysis.

2011-06-01 | E-GEOD-28091 | biostudies-arrayexpress

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Expression profiling of mouse glioma-initiating cell-like cells (GICs) and non-GICs

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Expression profiling of glioma initiating cells (GICs) in the sphere and differentiation conditions

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