Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

Transcription profiling of activated or unactivated T cells isolated from peripheral human leukocytes under gravity or vectorless gravity conditions


ABSTRACT: Peripheral blood leukocytes of three human donors (Donors 2, 4, and 5) were isolated from blood bank buffy-coat preparations by Ficoll-Hypaque density gradient centrifugation, and T-cells were purified with high affinity negative selection human CD3+ T-cell enrichment columns. The cells were resuspended in RPMI-1640 with 10% FCS with approximately 3-8 millions cells/mL at room temperature overnight. T-cells were then either unactivated (1g 0 hr), activated (1g 4 hr) with a final concentration of 5 mg/mL Con A + 4 mg/mL anti-CD28 antibody and incubated for 4 hours at 37°C, or loaded onto a RPM rotating at 60°/s (vg). T-cells loaded on the RPM were cultured for 2 hours prior to collection or activation to allow equilibration of cells to the new environment. After incubation, 1 mL of 4 M guanidinium isothiocyanate in 750 mM sodium citrate buffer, pH 7, with N-lauroylsarcosine and ß-mercaptoethanol was added to lyse cells and stabilize RNA.

ORGANISM(S): Homo sapiens

SUBMITTER: Boonyaratanakornkit Jim, 

PROVIDER: E-GEOD-1709 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

altmetric image

Publications

Key gravity-sensitive signaling pathways drive T cell activation.

Boonyaratanakornkit J B JB   Cogoli A A   Li C-F CF   Schopper T T   Pippia P P   Galleri G G   Meloni M A MA   Hughes-Fulford M M  

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 20051006 14


Returning astronauts have experienced altered immune function and increased vulnerability to infection during spaceflights dating back to Apollo and Skylab. Lack of immune response in microgravity occurs at the cellular level. We analyzed differential gene expression to find gravity-dependent genes and pathways. We found inhibited induction of 91 genes in the simulated freefall environment of the random positioning machine. Altered induction of 10 genes regulated by key signaling pathways was ve  ...[more]

Similar Datasets

2005-08-25 | GSE1709 | GEO
2012-06-20 | E-GEOD-38836 | biostudies-arrayexpress
2012-06-21 | GSE38836 | GEO
2014-08-23 | GSE60473 | GEO
2013-06-26 | E-GEOD-33801 | biostudies-arrayexpress
2019-10-15 | GSE134102 | GEO
2014-08-23 | E-GEOD-60473 | biostudies-arrayexpress
2010-05-28 | E-GEOD-21837 | biostudies-arrayexpress
2010-06-17 | E-GEOD-22387 | biostudies-arrayexpress
2018-04-20 | GSE113391 | GEO