Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of mouse fetal liver feeder layer and endothelial progenitor cells


ABSTRACT: While others have reported that fetal liver contains a population of endothelial progenitors based on expression of cell surface markers or culture assays, this is the first proof of a CD31+Sca1+ progenitor by demonstrating highly efficient in vivo angiogenesis and a direct connection to the host vasculature. We have developed a novel isolation method based on collagenase digestion and culture on a fetal liver-derived feeder layer and demonstrate that the feeder cells or their supernatants are required for endothelial progenitor survival and proliferation. Proteogenomic profiling of the endothelial progenitors and the feeder cells was done with tandem mass spectrometry proteomics using MudPIT and gene transcript expression profiling using high density DNA microarrays. This approach identified a number of gene transcripts, proteins and candidate growth factor pathways that are likely to be involved in endothelial progenitor growth, differentiation and angiogenesis.

ORGANISM(S): Mus musculus

SUBMITTER: Sunil Mathan Kurian 

PROVIDER: E-GEOD-1727 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Isolation and angiogenesis by endothelial progenitors in the fetal liver.

Cherqui Stephanie S   Kurian Sunil M SM   Schussler Olivier O   Hewel Johannes A JA   Yates John R JR   Salomon Daniel R DR  

Stem cells (Dayton, Ohio) 20050811 1


Endothelial progenitor cells (EPCs) have significant therapeutic potential. However, the low quantity of such cells available from bone marrow and their limited capacity to proliferate in culture make their use difficult. Here, we present the first definitive demonstration of the presence of true EPCs in murine fetal liver capable of forming blood vessels in vivo connected to the host's vasculature after transplantation. This population is particularly interesting because it can be obtained at h  ...[more]

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