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Undifferentiated mouse embryonic stem cells, differentiated nestin-positive cells and fibroblast feeder layer.

ABSTRACT: Embryonic stem (ES) cells and ES cell-derived progeny characterized by nestin expression (including neural progenitors) were studied (three independent experiments). The mouse ES cell line R1 was cultured on a feeder layer of mouse embryonic fibroblasts (FL). ES cells were differentiated into nestin-positive cells for 4+8 days and 4+11 days according to the differentiation protocol by Rolletschek et al. (Mechanisms of Development 105, 93-104, 2001). The study was performed with three independent cell cultures (= triplicates). RNA was prepared from both undifferentiated ES cells and ES cell-derived nestin-positive cells, and from fibroblast feeder cells. Keywords = ES cell Keywords = nestin positive cells Keywords = feeder layer Keywords: other

ORGANISM(S): Mus musculus

PROVIDER: GSE2375 | GEO | 2005/09/17

SECONDARY ACCESSION(S): PRJNA91531

REPOSITORIES: GEO

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Transcription profiling of mouse undifferentiated embryonic stem cells, differentiated nestin-positive cells and fibroblast feeder layer.

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