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Alternative splicing in HeLa cells after sodium butyrate treatment

ABSTRACT: We analyzed a role of histone deacetylases in alternative splicing regulation. Using human exon arrays we identified a list of 683 genes whose splicing changes after HDAC inhibition with sodium butyrate. 6 samples (3 nontreated controls and 3 sodium butyrate treated cells)

ORGANISM(S): Homo sapiens

SUBMITTER: David Stanek

PROVIDER: E-GEOD-17397 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Histone deacetylase activity modulates alternative splicing.

Hnilicová Jarmila J Hozeifi Samira S Dušková Eva E Icha Jaroslav J Tománková Tereza T Staněk David D

PloS one 20110202 2

There is increasing evidence to suggest that splicing decisions are largely made when the nascent RNA is still associated with chromatin. Here we demonstrate that activity of histone deacetylases (HDACs) influences splice site selection. Using splicing-sensitive microarrays, we identified ∼700 genes whose splicing was altered after HDAC inhibition. We provided evidence that HDAC inhibition induced histone H4 acetylation and increased RNA Polymerase II (Pol II) processivity along an alternatively ...[more]

PMID: 21311748

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Alternative splicing in HeLa cells after sodium butyrate treatment

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2011-02-03 | GSE17397 | GEO

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Use of microarray analyses to determine the effect of hyperoxia and hypoxia on gene expression in early oxygen-induced retinopathy (OIR).

Project description:Different inbred strains of rats differ in their susceptibility to OIR, an animal model of human retinopathy of prematurity. We examined gene expression profiles in Fischer 344 (F344, resistant to OIR) and Sprague Dawley (SD, susceptible to OIR) rats at the early time points of day 5 (in response to hyperoxia) and day 6 (in response to relative hypoxia) to identify gene pathways related to the underlying genetic cause of the phenotypic differences observed between strains. To examine gene expression changes in rat strains which are resistant and susceptible to OIR, four different experimental conditions were analysed: F344 cyclic hyperoxia (O2) exposed, F344 room air (RA) exposed, SD O2 exposed and SD RA exposed. Two samples of pooled RNA, comprising of 3 individual rats from 2 separate litters, was used for each experimental condition, for each time point of interest. Pooled RNA from age-matched room air-exposed rats were used as controls.

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Misregulation of alternative splicing of BIN1 leads to T-tubule alterations and muscle weakness in myotonic dystrophy

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2012-03-21 | E-GEOD-28095 | biostudies-arrayexpress

Expression changes in cultured endothelial cells upon infection with HHV-8

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2011-07-31 | E-GEOD-27136 | biostudies-arrayexpress

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