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Alternative splicing in HeLa cells after sodium butyrate treatment

ABSTRACT: We analyzed a role of histone deacetylases in alternative splicing regulation. Using human exon arrays we identified a list of 683 genes whose splicing changes after HDAC inhibition with sodium butyrate. 6 samples (3 nontreated controls and 3 sodium butyrate treated cells)

ORGANISM(S): Homo sapiens

SUBMITTER: David Stanek

PROVIDER: E-GEOD-17397 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Histone deacetylase activity modulates alternative splicing.

Hnilicová Jarmila J Hozeifi Samira S Dušková Eva E Icha Jaroslav J Tománková Tereza T Staněk David D

PloS one 20110202 2

There is increasing evidence to suggest that splicing decisions are largely made when the nascent RNA is still associated with chromatin. Here we demonstrate that activity of histone deacetylases (HDACs) influences splice site selection. Using splicing-sensitive microarrays, we identified ∼700 genes whose splicing was altered after HDAC inhibition. We provided evidence that HDAC inhibition induced histone H4 acetylation and increased RNA Polymerase II (Pol II) processivity along an alternatively ...[more]

PMID: 21311748

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