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Innervated and 2 month Denervated Rat EDL muscles

ABSTRACT: The right legs of 8 Brown-Norway male rats were denervated by a high sciatic nerve section in the hip region of the hind limb.Two months after denervation (6 months of age), extensor digitorum longus (EDL) muscles were removed from the operated legs. The EDL muscles from 8 age-matched non-operated rats served as innervated controls. Total RNA was isolated, labeled cDNA was prepared and hybridized to the Rat Atlas 1.2 Array II membranes (Clontech Laboratories, Palo Alto, CA).

ORGANISM(S): Rattus norvegicus

SUBMITTER: Tatiana Kostrominova

PROVIDER: E-GEOD-1741 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Comparison of gene expression of 2-mo denervated, 2-mo stimulated-denervated, and control rat skeletal muscles.

Kostrominova Tatiana Y TY Dow Douglas E DE Dennis Robert G RG Miller Richard A RA Faulkner John A JA

Physiological genomics 20050419 2

Loss of innervation in skeletal muscles leads to degeneration, atrophy, and loss of force. These dramatic changes are reflected in modifications of the mRNA expression of a large number of genes. Our goal was to clarify the broad spectrum of molecular events associated with long-term denervation of skeletal muscles. A microarray study compared gene expression profiles of 2-mo denervated and control extensor digitorum longus (EDL) muscles from 6-mo-old rats. The study identified 121 genes with in ...[more]

PMID: 15840640

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Innervated and 2 month Denervated Rat EDL muscles

Project description:The right legs of 8 Brown-Norway male rats were denervated by a high sciatic nerve section in the hip region of the hind limb.Two months after denervation (6 months of age), extensor digitorum longus (EDL) muscles were removed from the operated legs. The EDL muscles from 8 age-matched non-operated rats served as innervated controls. Total RNA was isolated, labeled cDNA was prepared and hybridized to the Rat Atlas 1.2 Array II membranes (Clontech Laboratories, Palo Alto, CA). Keywords: other

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Transcriptional responses to chronic oxidative stress in skeletal muscle

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2011-04-26 | E-GEOD-28837 | biostudies-arrayexpress

Transcriptional responses to chronic oxidative stress in skeletal muscle

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