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MiRNA Profiling from total blood of lung cancer and control samples

ABSTRACT: This project aims at the detection of specific patterns of miRNAs in peripheral blood samples of lung cancer patients. As controls, blood of donors without known affection have been tested. Using the miRNA patterns we hope to detect a diagnostic pattern for the non-invasive diagnosis of non-small cell lung carcinoma. In this study, we compared 17 lung cancer samples to 19 control samples using the sanger miRBAse 12.0 miRNA biochip manufactured by febit. Samples were analyzed with the Geniom Realtime Analyzer (GRTA, febit gmbh, Heidelberg, Germany) using the Geniom Biochip miRNA Homo sapiens. Each array contains 7 replicates of 866 miRNAs and miRNA star sequences as annotated in the Sanger miRBase 12.0. To benchmark the platform, we tested technical replicates using bought total RNA of brain and liver samples (ambion), achieving a correlation of 0.97.

ORGANISM(S): Homo sapiens

SUBMITTER: Hanno Huwer

PROVIDER: E-GEOD-17681 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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miRNAs in lung cancer - studying complex fingerprints in patient's blood cells by microarray experiments.

Keller Andreas A Leidinger Petra P Borries Anne A Wendschlag Anke A Wucherpfennig Frank F Scheffler Matthias M Huwer Hanno H Lenhof Hans-Peter HP Meese Eckart E

BMC cancer 20091006

<h4>Background</h4>Deregulated miRNAs are found in cancer cells and recently in blood cells of cancer patients. Due to their inherent stability miRNAs may offer themselves for blood based tumor diagnosis. Here we addressed the question whether there is a sufficient number of miRNAs deregulated in blood cells of cancer patients to be able to distinguish between cancer patients and controls.<h4>Methods</h4>We synthesized 866 human miRNAs and miRNA star sequences as annotated in the Sanger miRBase ...[more]

PMID: 19807914

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