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Mycobacterium tuberculosis TraSH experiment: Growth in wild type C57BL/6J versus iNOS-/- mice


ABSTRACT: A M. tuberculosis transposon library was used to infect WT and iNOS-/- mice. Surviving mutants were recovered from spleens, genomic DNA was extracted, and labeled probes were synthesized from transposon ends. Probes from each WT mouse were hybridized with probes from a similar iNOS-/- mouse. Two-condition experiment, Growth in WT vs. iNOS-/- mice. Biological replicates: TraSH probe made from 5 wild type and 5 iNOS-/- mice after 3 weeks of infection and from 8 wild type and 8 iNOS-/- mice after 4 weeks of infection. Technical replicates: TraSH probe was synthesized twice from each mouse and dyes were swapped. One array contains probe from one iNOS-/- and one WT mouse. Each biological replicate has two arrays, representing technical replicate dye swaps from one iNOS-/- and one WT mouse.

ORGANISM(S): Mycobacterium tuberculosis

SUBMITTER: Christopher Sassetti 

PROVIDER: E-GEOD-17706 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Phthiocerol dimycocerosate transport is required for resisting interferon-gamma-independent immunity.

Murry Jeffrey P JP   Pandey Amit K AK   Sassetti Christopher M CM   Rubin Eric J EJ  

The Journal of infectious diseases 20090901 5


Nitric oxide (NO), which is an important component of immunity to Mycobacterium tuberculosis, has both cytotoxic and immune regulatory functions. We examined the way that this molecule interacts with M. tuberculosis in vivo by screening for bacterial mutations that alter growth in mice that are unable to produce inducible NO synthase (iNOS), the dominant source of NO during infection. We found that very few bacterial genes appeared to be specifically required for resistance to NO in vivo. Instea  ...[more]

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