Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Gene expression profiles in rat lung following intratracheal instillation with C60 fullerene particles

ABSTRACT: Gene expression profiling of the rat lung following intratracheal instillation with C60 fullerene particles was employed to gain insights into these molecular events. Groups of nine-week-old male Wistar rats (n= 4-6 per group/ time point) were intratracheally instilled with C60 fullerene suspended in 0.4 ml distilled water including 0.8% Tween-80 as a single injection (0.1 mg, 0.2 mg (LD: low doses) and 1.0 mg (HD: high dose) C60 fullerene/ rat). Control groups received 0.8 % Tween-80 (vehicle control). After intratracheal instillation treatment, rats were housed within polycarbonate cages at a controlled temperature of 22 °C with a chow diet ad libitum, and dissected at 3 days, 1 week, 1 month, 3 month, and 6 month post-instillation. Right lungs of anesthetized rats were perfused with physiological saline, excised, and used for DNA microarray analysis.

ORGANISM(S): Rattus norvegicus

SUBMITTER: Katsuhide Fujita

PROVIDER: E-GEOD-17747 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Gene expression profiles in rat lung following intratracheal instillation with single-wall carbon nanotubes (SWCNTs)

Project description:Gene expression profiling of the rat lung following intratracheal instillation with SWCNTs was employed to gain insights into these molecular events. We attempted to characterize time-dependent changes in the gene expression until 754 days after intratracheal instillation with SWCNTs suspensions at 0.2 mg (L-SWCNT) and 0.4 mg (H-SWCNT) injected dose per rat, and to identify the shift from the acute-phase to the chronic-phase phase on the basis of evaluation at the molecular level. Groups of nine-week-old male Wistar rats (n= 6 per group/ time point) were intratracheally instilled with single-wall carbon nanotubes (SWCNTs) suspended in 0.4 ml distilled water including 0.1% Triton X-100 as a single injection 0.1 mg (L-SWCNT) and 0.4 mg (H-SWCNT) SWCNTs/ rat). Control groups received 0.1% Triton X-100 (vehicle control). After intratracheal instillation treatment, rats were housed within polycarbonate cages at a controlled temperature of 22 M-BM-0C with a chow diet ad libitum, and dissected at 3 days, 7 days, 30 days, 90 days, 180 days, 365 days and 754 days post-instillation. Right lungs of anesthetized rats were perfused with physiological saline, excised, and used for DNA microarray analysis.

2013-09-07 | E-GEOD-50664 | biostudies-arrayexpress

Gene expression profiles in rat lung following intratracheal instillation with impurity-free single-wall carbon nanotubes

Project description:To further development of our gene expression approach to assess the effects of manufactured nanomaterials at the transcriptional level, we have employed whole genome microarray expression profiling as a discovery platform to identify genes with the potential to distinguish characterization of physicochemical properties of impurity-free single-wall carbon nanotubes (SWCNTs). We have prepared two types of dispersed the SWCNTs, namely relatively small bundles and a short linear shape (CNT-1) and large bundles and a long linear shape (CNT-2), and attempted to characterize time-dependent changes in the gene expression of lung tissues until 90 days after intratracheal instillation with SWCNTs suspensions at 0.4 mg injected dose per rat. Groups of nine-week-old male Wistar rats (n= 4 per group/ time point) were intratracheally instilled with single-wall carbon nanotubes (SWCNTs) suspended in 0.4 ml of 1.0mg/mL bovine serum albumin (BSA) as a single injection 0.4 mg SWCNTs/ rat. Control groups received 1.0mg/mL BSA (vehicle control). After intratracheal instillation treatment, rats were housed within polycarbonate cages at a controlled temperature of 22 M-BM-0C with a chow diet ad libitum, and dissected at 1day, 3 days, 7 days, 30 days, and 90 days post-instillation. Right lungs of anesthetized rats were perfused with physiological saline, excised, and used for DNA microarray analysis.

2014-09-17 | E-GEOD-61483 | biostudies-arrayexpress

Exposure to urban particulate matter (EHC-93)

Project description:We isolated total lung RNA from spontaneously hypertensive male rats 2–40 h after exposure to reference EHC-93. Three animals were exposed to EHC-93 in saline(10 mg/kg body weight) or saline at each time via intratracheal instillation or no instillation at all. RNA from different times was pooled for array hybridization as indicated. Keywords: time-course

2004-11-01 | GSE1796 | GEO

Transcription profiling of lung RNA from spontaneously hypertensive male rats after exposure to urban particulate matter (EHC-93)

Project description:We isolated total lung RNA from spontaneously hypertensive male rats 2â40 h after exposure to reference EHC-93. Three animals were exposed to EHC-93 in saline(10 mg/kg body weight) or saline at each time via intratracheal instillation or no instillation at all. RNA from different times was pooled for array hybridization as indicated.

2008-06-12 | E-GEOD-1796 | biostudies-arrayexpress

Pulmonary miRNA expression in C57BL/6 Bom Tac mice (dams) intratracheally instilled with Printex 90 carbon black nanoparticles

Project description:miRNAs were measured in pregant mice exposed to vehicle control (nanopure water) or a cumulative dose of 0.268 mg Printex 90 in vehicle by intratracheal instillation and sacrificed at weaning (26-27 days after exposure). Pregnant mice were exposed to vehicle control (nanopure water) or a cumulative dose of 0.268 mg Printex 90 in vehicle by intratracheal instillation. Doses of 0.067 mg were delivered 4 times in pregnant females on gestational days 7, 10, 15 and 18. Mice were sacrificed at weaning (26-27 days post-exposure).

2012-04-01 | E-GEOD-36170 | biostudies-arrayexpress

Gene expression profiles in rat lung following intratracheal instillation with single-wall carbon nanotubes

Project description:Gene expression profiling of the rat lung following intratracheal instillation with single-wall carbon nanotubes (SWCNTs) was employed to gain insights into these molecular events. We attempted to characterize time-dependent changes in the gene expression until 754 days after intratracheal instillation with SWCNTs suspensions at 0.2 mg (L-SWCNT) and 0.4 mg (H-SWCNT) injected dose per rat, and to identify the shift from the acute-phase to the chronic-phase phase on the basis of evaluation at the molecular level.

2013-09-07 | GSE50664 | GEO

Intratracheal instillation of quaternary ammonium compounds in rats

Project description:Intratracheal instillation of quaternary ammonium compounds in rats

| PRJNA1096695 | ENA

Pulmonary miRNA expression in C57BL/6 Bom Tac mice (dams) intratracheally instilled with Printex 90 carbon black nanoparticles

2012-04-02 | GSE36170 | GEO

Pulmonary miRNA expression in C57BL/6 non-pregnant female mice intratracheally instilled with Printex 90 carbon black nanoparticles

Project description:miRNAs were measured in mice exposed to vehicle control (0.9% NaCl MilliQ water containing 10% v/v acellular BAL) or 0.162 mg Printex 90 in vehicle by intratracheal instillation and sacrificed 1, 3 and 28 days after exposure.

2012-04-02 | GSE36171 | GEO

Murine bronco alveolar lavage fluid from wild type and mannose receptor knock out mice, unchallenged and LPS challenged

Project description:A comparative analysis of the protein content in bronco alveolar lavage fluid from wild type and mannose receptor knock-out littermate mice. This includes both an unchallenged mice and mice subjected to intratracheal LPS instillation to induce injury and inflammation.

2024-04-17 | PXD049201 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data