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Pulmonary miRNA expression in C57BL/6 Bom Tac mice (dams) intratracheally instilled with Printex 90 carbon black nanoparticles

ABSTRACT: miRNAs were measured in pregant mice exposed to vehicle control (nanopure water) or a cumulative dose of 0.268 mg Printex 90 in vehicle by intratracheal instillation and sacrificed at weaning (26-27 days after exposure). Pregnant mice were exposed to vehicle control (nanopure water) or a cumulative dose of 0.268 mg Printex 90 in vehicle by intratracheal instillation. Doses of 0.067 mg were delivered 4 times in pregnant females on gestational days 7, 10, 15 and 18. Mice were sacrificed at weaning (26-27 days post-exposure).

ORGANISM(S): Mus musculus

SUBMITTER: Andrew Williams

PROVIDER: E-GEOD-36170 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Hepatic and pulmonary toxicogenomic profiles in mice intratracheally instilled with carbon black nanoparticles reveal pulmonary inflammation, acute phase response, and alterations in lipid homeostasis.

Bourdon Julie A JA Halappanavar Sabina S Saber Anne T AT Jacobsen Nicklas R NR Williams Andrew A Wallin Håkan H Vogel Ulla U Yauk Carole L CL Yauk Carole L CL

Toxicological sciences : an official journal of the Society of Toxicology 20120329 2

Global pulmonary and hepatic messenger RNA profiles in adult female C57BL/6 mice intratracheally instilled with carbon black nanoparticles (NPs) (Printex 90) were analyzed to identify biological perturbations underlying systemic responses to NP exposure. Tissue gene expression changes were profiled 1, 3, and 28 days following exposure to 0.018, 0.054, and 0.162 mg Printex 90 alongside controls. Pulmonary response was marked by increased expression of inflammatory markers and acute phase response ...[more]

PMID: 22461453

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Pulmonary miRNA expression in C57BL/6 Bom Tac mice (dams) intratracheally instilled with Printex 90 carbon black nanoparticles

Project description:miRNAs were measured in pregant mice exposed to vehicle control (nanopure water) or a cumulative dose of 0.268 mg Printex 90 in vehicle by intratracheal instillation and sacrificed at weaning (26-27 days after exposure).

2012-04-02 | GSE36170 | GEO

Pulmonary miRNA expression in C57BL/6 non-pregnant female mice intratracheally instilled with Printex 90 carbon black nanoparticles

Project description:miRNAs were measured in mice exposed to vehicle control (0.9% NaCl MilliQ water containing 10% v/v acellular BAL) or 0.162 mg Printex 90 in vehicle by intratracheal instillation and sacrificed 1, 3 and 28 days after exposure. C57BL/6 mice were exposed to 0.000 or 0.162 mg Printex 90 and sacrificed 1, 3 and 28 days after exposure. Individual total RNA enriched with small RNA from 6 mice per treatment group (control and 0.162mg) was hybridized to 8 x 15K Agilent miRNA slides.

2012-04-01 | E-GEOD-36171 | biostudies-arrayexpress

Cardiac miRNA expression (random section containing both ventricles) in C57BL/6 mice intratracheally instilled with Printex 90 carbon black nanoparticles

Project description:C57BL/6 mice were exposed to vehicle or 0.162 mg carbon black nanoparticles by intratracheal installation and were sacrificed 1, 3 and 28 days post-exposure. Gene expression was investigated in two sets of mice treated according to the identical exposure protocol. MicroRNAs were investigated in the second set of mice. C57BL/6 mice were exposed to 0.000 or 0.162 mg Printex 90 and were sacrificed 1, 3 and 28 days after exposure. Individual total RNA enriched with small RNA from 6 mice per treatment group (control and 0.162mg) was hybridized to 8 x 15K Agilent miRNA slides.

2013-05-02 | E-GEOD-37890 | biostudies-arrayexpress

Pulmonary miRNA expression in C57BL/6 non-pregnant female mice intratracheally instilled with Printex 90 carbon black nanoparticles

2012-04-02 | GSE36171 | GEO

Pulmonary miRNA expression in C57BL/6 mice intratracheally instilled with Printex 90 carbon black nanoparticles

Project description:This SuperSeries is composed of the following subset Series: GSE36170: Pulmonary miRNA expression in C57BL/6 Bom Tac mice (dams) intratracheally instilled with Printex 90 carbon black nanoparticles GSE36171: Pulmonary miRNA expression in C57BL/6 non-pregnant female mice intratracheally instilled with Printex 90 carbon black nanoparticles Refer to individual Series

2012-04-01 | E-GEOD-36199 | biostudies-arrayexpress

Gene expression profiles in rat lung following intratracheal instillation with single-wall carbon nanotubes (SWCNTs)

Project description:Gene expression profiling of the rat lung following intratracheal instillation with SWCNTs was employed to gain insights into these molecular events. We attempted to characterize time-dependent changes in the gene expression until 754 days after intratracheal instillation with SWCNTs suspensions at 0.2 mg (L-SWCNT) and 0.4 mg (H-SWCNT) injected dose per rat, and to identify the shift from the acute-phase to the chronic-phase phase on the basis of evaluation at the molecular level. Groups of nine-week-old male Wistar rats (n= 6 per group/ time point) were intratracheally instilled with single-wall carbon nanotubes (SWCNTs) suspended in 0.4 ml distilled water including 0.1% Triton X-100 as a single injection 0.1 mg (L-SWCNT) and 0.4 mg (H-SWCNT) SWCNTs/ rat). Control groups received 0.1% Triton X-100 (vehicle control). After intratracheal instillation treatment, rats were housed within polycarbonate cages at a controlled temperature of 22 M-BM-0C with a chow diet ad libitum, and dissected at 3 days, 7 days, 30 days, 90 days, 180 days, 365 days and 754 days post-instillation. Right lungs of anesthetized rats were perfused with physiological saline, excised, and used for DNA microarray analysis.

2013-09-07 | E-GEOD-50664 | biostudies-arrayexpress

Gene expression profiles in rat lung following intratracheal instillation with C60 fullerene particles

Project description:Gene expression profiling of the rat lung following intratracheal instillation with C60 fullerene particles was employed to gain insights into these molecular events. Groups of nine-week-old male Wistar rats (n= 4-6 per group/ time point) were intratracheally instilled with C60 fullerene suspended in 0.4 ml distilled water including 0.8% Tween-80 as a single injection (0.1 mg, 0.2 mg (LD: low doses) and 1.0 mg (HD: high dose) C60 fullerene/ rat). Control groups received 0.8 % Tween-80 (vehicle control). After intratracheal instillation treatment, rats were housed within polycarbonate cages at a controlled temperature of 22 °C with a chow diet ad libitum, and dissected at 3 days, 1 week, 1 month, 3 month, and 6 month post-instillation. Right lungs of anesthetized rats were perfused with physiological saline, excised, and used for DNA microarray analysis.

2009-08-26 | E-GEOD-17747 | biostudies-arrayexpress

Hepatic gene expression in C57BL/6 mice intratracheally instilled with Printex 90 carbon black nanoparticles

Project description:We investigated hepatic mRNA profiles of female mice exposed to 0.162 mg Printex 90 carbon black nanoparticles by single intra-tracheal instillation. We examined responses to this dose 1, 3 and 28 days after exposure, alongside respective controls. We show that genes part of the 3-hydroxy-3-methyl-glutaryl-CoA reductase pathway to be up-regulated by exposure to Printex 90.

2012-04-13 | GSE35192 | GEO

Gene expression profiles in rat lung following intratracheal instillation with single-wall carbon nanotubes

Project description:Gene expression profiling of the rat lung following intratracheal instillation with single-wall carbon nanotubes (SWCNTs) was employed to gain insights into these molecular events. We attempted to characterize time-dependent changes in the gene expression until 754 days after intratracheal instillation with SWCNTs suspensions at 0.2 mg (L-SWCNT) and 0.4 mg (H-SWCNT) injected dose per rat, and to identify the shift from the acute-phase to the chronic-phase phase on the basis of evaluation at the molecular level.

2013-09-07 | GSE50664 | GEO

Lack of hepatic response of microRNA in mice following DB[a,h]A exposure (Agilent miRNA)

Project description:Dibenzo[a,h]anthracene DB[a,h]A is a polycyclic aromatic hydrocarbon potent carcinogen. Few studies have investigated the role of DB[a,h]A on mRNA and miRNA expression. In this study a 10-week old male MutaTM Mouse were exposed to 6.25, 12.5, and 25 mg/kg/day DB[a,h]A by oral gavage for 28 consecutive days. DNA adducts were detected in the livers at each DB[a,h]A dose tested, and a dose-dependent increase in lacZ mutants was observed in the same samples. MAANOVA analysis revealed minor changes in the mRNA expression for the two lowest doses. Differential expression of 19 up-regulated and 22 down-regulated transcripts with fold-change > 1.5 (FDR-adjusted P < 0.05) were identified in the 6.25 mg/kg/day DB[a,h]A treatment group. For the 12.5 mg/kg/day treatment group 13 transcripts were up-regulated and 32 down-regulated (FDR-adjusted P < 0.05 and fold-change > 1.5). Major effect on mRNA expression resulted from exposure to the highest dose (25 mg/kg/day) of DB[a,h]A with 135 up-regulated and 104 down-regulated genes with fold-change > 1.5 (FDR-adjusted P < 0.05). The significantly regulated genes are involved in circadian rhythm, drug metabolism, glucose metabolism, cholestrol and lipid metabolism, immune response, cell cycle, and apoptosis. We also investigated miRNA response to the three doses of DB[a,h]A. MiRNA expression was relatively unaffected. Only miR-34a showed significant (FDR-adjusted P < 0.05) up-regulation with a fold change above 1.3-fold. RNA samples from 5 control and 4-5 mice per treatment group (6.25mg/kg, 12.50mg/kg, and 25mg/kg) containing 100 ng were labelled using AgilentM-bM-^@M-^Ys miRNA complete labelling and Hyb Kit (Agilent Tech, Mississauga, ON, Canada).

2013-07-02 | E-GEOD-35195 | biostudies-arrayexpress

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