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Transcription profiling by array of mouse myotubes grown under control and starvation conditions

ABSTRACT: C2C12 mouse myoblasts (American Type Culture Collection, Manassas, VA) were maintained in growth medium (DMEM supplemented with 10% Fetal Bovine serum, Invitrogen Corporation, Carlsbad, CA) in 5% CO2. Cells were plated at a density of 40-50% in 100mm dishes. When cells reached ~90% confluency 24 hours later, they were switched to differentiation media (DMEM containing 2% horse serum; GIBCO, Invitrogen Corporation, Carlsbad, CA) which was subsequently changed every 48 hours. All cells were allowed to differentiate for 4 days with feeding every two days (day 0, 2, and 4). Beginning at day 4, control cells were re-fed every 48 hours whereas experimental cells were not re-fed. After an additional 4 days (8 days post differentiation), cells were harvested and total RNA was isolated from control and starved (non re-fed cells)

ORGANISM(S): Mus musculus

SUBMITTER: Susan Kandarian

PROVIDER: E-GEOD-1776 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Transcriptional profile of a myotube starvation model of atrophy.

Stevenson Eric J EJ Koncarevic Alan A Giresi Paul G PG Jackman Robert W RW Kandarian Susan C SC

Journal of applied physiology (Bethesda, Md. : 1985) 20041217 4

Skeletal muscle wasting is a pervasive phenomenon that can result from a wide range of pathological conditions as well as from habitual muscular inactivity. The present work describes a cell-culture condition that induces significant atrophy in skeletal muscle C2C12 myotubes. The failure to replenish differentiation media in mature myotubes leads to rapid atrophy (53% in diameter), which is referred to here as starvation. Affymetrix microarrays were used to develop a transcriptional profile of c ...[more]

PMID: 15608089

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