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Effect of Exportin-5 expression on Mouse fibroblasts in cell cycle re-entry phase

ABSTRACT: To analyze the effect of Exportin-5 expression on the MEF cells in cell cycle re-entry phase, we have employed whole genome microarray expression profiling on the MEF cells in cell cycle re-entry phase with and without down regulation of Exportin-5 gene. Mouse MEF cells were transfected with 60nM of siRNA targeting either Exportin-5 or negative control, and incubated for 12 hours. After incubation, cells were starved with DMEM containing 0.2% FCS for 48 hours and re-fed with DMEM containing 20%FCS for 24 hours, along with second siRNA transfection. Two independant expreiments were preformed.

ORGANISM(S): Mus musculus

SUBMITTER: Yoko Yuzawa

PROVIDER: E-GEOD-25498 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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