Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

Effect of simultaneous activation of TGFbeta and TLR4 pathway in human monocytes


ABSTRACT: To better understand the interaction of TGFbeta and Toll-like-receptor 4 (TLR4) signaling in monocytes, we performed microarray analysis. We identified a unique set of genes whose expressions in monocytes were regulated by simultaneous stimulation of TLR4 and TGFβ signaling. THP-1 cells, a human monocytic cell line, were stimulated for 24 hours with PBS as control, LPS (100ng/ml), TGFbeta1 (1ng/ml) or LPS (100ng/ml) + TGFbeta1 (1ng/ml). After 24 hours of stimulation, total RNA was isolated and prepared for genome wide analyses using Illumina HumanRef8 V3.0 Beadchips. In total 32 arrays were analyzed including 8 samples of PBS-stimulated monocytes, 8 samples of LPS-stimulated monocytes, 8 samples of TGFbeta1 stimulated monocytes and 8 samples of monocytes stimulated with LPS + TGFbeta1.

ORGANISM(S): Homo sapiens

SUBMITTER: Karlijn van Keulen 

PROVIDER: E-GEOD-17909 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

Similar Datasets

2014-12-03 | E-MEXP-3931 | biostudies-arrayexpress
2018-09-01 | GSE97260 | GEO
2014-07-11 | E-GEOD-55375 | biostudies-arrayexpress
2020-05-26 | GSE99386 | GEO
2020-05-26 | GSE99376 | GEO
2010-12-22 | E-GEOD-19627 | biostudies-arrayexpress
2022-08-25 | GSE157182 | GEO
2011-07-30 | E-GEOD-31067 | biostudies-arrayexpress
2011-07-30 | E-GEOD-31066 | biostudies-arrayexpress
2015-06-11 | ST000209 | MetabolomicsWorkbench