Dataset Information

Acetylomics on rhein treated NLRP3 activated J774A.1 macrophages

ABSTRACT: The aim of this project is to observe the effect of 5 μM rhein treatment on the acetylation level of NLRP3 activated macrophages. We primed J774A.1 cells with 100ng/mL LPS for 12 hours followed by 5μM rhein treatment for 30 minutes. Finally, the cells were stimulated with 4 mM ATP for 30 min to trigger the inflammasome assembly. Cell lysates were collected after ATP stimulation by cell scrapers, and acetylomics were performed as described below.

INSTRUMENT(S): timsTOF

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Macrophage

SUBMITTER: Ruonan Zhou

LAB HEAD: Wenbin Shang

PROVIDER: PXD044992 | Pride | 2024-10-17

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	RE303LPAcB1_M1_Slot1-18_1_20751.d.zip	Other
	RE303LPAcB1_M2_Slot1-19_1_20753.d.zip	Other
	RE303LPAcB1_M3_Slot1-20_1_20755.d.zip	Other
	RE303LPAcB1_R1_Slot1-21_1_20757.d.zip	Other
	RE303LPAcB1_R2_Slot1-22_1_20759.d.zip	Other

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Publications

Rhein targets macrophage SIRT2 to promote adipose tissue thermogenesis in obesity in mice.

Zhou Ruo-Nan RN Zhu Zi-Wei ZW Xu Ping-Yuan PY Shen Li-Xuan LX Wang Ziwei Z Xue Ying-Ying YY Xiang Ying-Ying YY Cao Yue Y Yu Xi-Zhong XZ Zhao Juan J Jin Yu Y Yan Jing J Yang Qin Q Fang Peng-Hua PH Shang Wen-Bin WB

Communications biology 20240816 1

Rhein, a component derived from rhubarb, has been proven to possess anti-inflammatory properties. Here, we show that rhein mitigates obesity by promoting adipose tissue thermogenesis in diet-induced obese mice. We construct a macrophage-adipocyte co-culture system and demonstrate that rhein promotes adipocyte thermogenesis through inhibiting NLRP3 inflammasome activation in macrophages. Moreover, clues from acetylome analysis identify SIRT2 as a potential drug target of rhein. We further verify ...[more]

PMID: 39152196

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