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Sex-related transcriptional differences in Day 7 bovine in vitro produced blastocysts

ABSTRACT: Sexual dimorphism in mammals is mostly attributable to sex-related hormonal differences in fetal and adult tissues; however, this may not be the sole determinant. Though genetically-identical for autosomal chromosomes, male and female preimplantation embryos could display sex-specific transcriptional regulation which can only be attributted to the differences in sexual chromosome dosage. We used microarrays to analyze sex-related transcriptional differences at the blastocyst stage. Day 7 bovine in vitro produced bovine blastocysts produced with sorted semen from 3 different bulls. Pooled RNA from 60 blastocysts of one sex and produced with one bull was used per chip. Three replicates of each sex per bull. In total, 18 Bovine GeneChip (Affymetrix) were used (3 replicates X 3 bulls X 2 sexes).

ORGANISM(S): Bos taurus

SUBMITTER: Alfonso GutiÃ©rrez-AdÃ¡n

PROVIDER: E-GEOD-17921 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Sex determines the expression level of one third of the actively expressed genes in bovine blastocysts.

Bermejo-Alvarez P P Rizos D D Rath D D Lonergan P P Gutierrez-Adan A A

Proceedings of the National Academy of Sciences of the United States of America 20100204 8

Although genetically identical for autosomal Chrs (Chr), male and female preimplantation embryos could display sex-specific transcriptional regulation. To illustrate sex-specific differences at the mRNA level, we compared gene-expression patterns between male and female blastocysts by DNA microarray comparison of nine groups of 60 bovine in vitro-produced blastocysts of each sex. Almost one-third of the transcripts detected showed sexual dimorphism (2,921 transcripts; false-discovery rate, P < 0 ...[more]

PMID: 20133684

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