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Decoding bull fertility in vitro: A proteomics exploration from sperm to blastocyst

ABSTRACT: Embryo development, In vitro fertilization, Bull fertility, Polyspermy, Proteomics Project description: The correlation between sperm traits, fertilization and embryonic development outcomes were investigated by means of functional in vitro assays and mass spectrometry-based proteomics experiments. Spermatozoa from four independent bulls, as well as approx.. 20 cells at 2-cell embryonic stages and full blastocysts (approx. 250 cells) derived from in vitro fertilization of oocytes with spermatozoa from these foru bulls were analyzed by MS-based proteomics.

INSTRUMENT(S): Orbitrap Exploris 240, timsTOF SCP

ORGANISM(S): Bos Taurus (bovine)

TISSUE(S): Spermatozoon, Embryo, Sperm, Early Embryonic Cell, Oocyte

SUBMITTER: Marcel Bühler

LAB HEAD: Kris Gevaert

PROVIDER: PXD054561 | Pride | 2025-03-28

REPOSITORIES: Pride

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			Action	DRS
	0602_UniversalContaminants.fasta	Fasta
	CMB-1435DIA_UPRC_9913X00899_1p_Ionopticks3_PM3_CMB-1435_KRGEV_1.raw	Raw
	CMB-1435DIA_UPRC_9913X00901_1p_Ionopticks3_PM3_CMB-1435_KRGEV_2.raw	Raw
	CMB-1435DIA_UPRC_9913X00903_1p_Ionopticks3_PM3_CMB-1435_KRGEV_3.raw	Raw
	CMB-1435DIA_UPRC_9913X00905_1p_Ionopticks3_PM3_CMB-1435_KRGEV_4.raw	Raw

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