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Transcriptomic analysis of maize developing earshoot, IBMRIL X IHP1 hybrid

ABSTRACT: Maize earshoot is a metabolic sink espcially related to nitrogen metabolism. Studies on the transcriptomic and metabolic changes occuring in earshoot can provide interesting answers about the nitrogen metabolic potential of the maize variety under study. Intermated B73 X Mo17 recombinant inbred lines (IBMRIL) belong to a predominantly used mapping population. Illinois high protein (IHP) line is a metabolic extreme with respect to nitrogen mebolism with around 30% seed protein. Developing earshoots from this genotype grown at nitrogen-deficient and nitrogen-sufficient conditions were sampled, processed and analyzed through microarray technology. IBMRIL population was crossed to IHP to find the allelic interaction related to nitrogen metabolism. Microarray experiment was conducted using the developing earshoots from IBMRIL X IHP1 lines that deffered in their grain yield significantly. Keywords: Yield response Technical replicates, dye swaps, technical replicates of dye swaps, no biological replicates

ORGANISM(S): Zea mays

SUBMITTER: Stephen Moose

PROVIDER: E-GEOD-17953 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Genetic factors that respond to N in maize leaf tissue

Project description:Genotype and nitrogen-dosage effect on maize leaves collected at V8 leaf stage B73 is a model maize genotype while Illinois high protein line (IHP) is a metabolic extreme selected for higher grain protein concentration. It is a well known fact that the leaves serve as source and earshoot as a sink. Microarray analysis of V8 leaf collected from B73 and IHP genotypes grown at vairable nitrogen applications. Keywords: Genotype and N-treatment response Technical replicates, dye swaps, technical replicates of dye swaps, no biological replicates

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Transcriptomic analysis of maize developing earshoot, B73 X Mo17 hybrid

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Transcriptomic analysis of maize developing earshoot, IBMRIL X IHP1 hybrid

Project description:Maize earshoot is a metabolic sink espcially related to nitrogen metabolism. Studies on the transcriptomic and metabolic changes occuring in earshoot can provide interesting answers about the nitrogen metabolic potential of the maize variety under study. Intermated B73 X Mo17 recombinant inbred lines (IBMRIL) belong to a predominantly used mapping population. Illinois high protein (IHP) line is a metabolic extreme with respect to nitrogen mebolism with around 30% seed protein. Developing earshoots from this genotype grown at nitrogen-deficient and nitrogen-sufficient conditions were sampled, processed and analyzed through microarray technology. IBMRIL population was crossed to IHP to find the allelic interaction related to nitrogen metabolism. Microarray experiment was conducted using the developing earshoots from IBMRIL X IHP1 lines that deffered in their grain yield significantly. Keywords: Yield response

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Transcriptomic analysis of maize developing earshoot, B73 X Mo17 hybrid

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2023-04-25 | GSE227472 | GEO

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