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Transcriptome analysis of 16 DAP seeds of Illinois protein lines.

ABSTRACT: Illinois protein lines (IPL) comprise lines known as Illinois high protein (IHP), Illinois low protein (ILP), Illinois reverse-low protein (IRLP) and Illinois reverse-high protein (IRHP) lines. These lines represent over a century old experiment containing lines that represent various levels of nitrogen metabolism potential as a function of their grain protein concentration. Microarray experiment was conducted using 16 DAP seeds from the above mentioned four IPLs. Keywords: Genotype response Technical replicates, dye swaps, technical replicates of dye swaps, no biological replicates

ORGANISM(S): Zea mays

SUBMITTER: Stephen Moose

PROVIDER: E-GEOD-18006 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Genotype changes in 8DAP leaf

Project description:Genotype dependent gene expression changes in the leaves of Illinois protein lines at 8 days after pollination (DAP). Illinois protein lines (IPL) comprise lines known as Illinois high protein (IHP), Illinois low protein (ILP), Illinois reverse-low protein (IRLP) and Illinois reverse-high protein (IRHP) lines. These lines represent over a century old experiment containing lines that represent various levels of nitrogen metabolism potential as a function of their grain protein concentration. Microarray experiment was conducted using 8 DAP leaves from the above mentioned four IPLs. Keywords: Genotype response Technical replicates, dye swaps, technical replicates of dye swaps, no biological replicates

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Genetic factors that respond to N in maize leaf tissue

Project description:Genotype and nitrogen-dosage effect on maize leaves collected at V8 leaf stage B73 is a model maize genotype while Illinois high protein line (IHP) is a metabolic extreme selected for higher grain protein concentration. It is a well known fact that the leaves serve as source and earshoot as a sink. Microarray analysis of V8 leaf collected from B73 and IHP genotypes grown at vairable nitrogen applications. Keywords: Genotype and N-treatment response Technical replicates, dye swaps, technical replicates of dye swaps, no biological replicates

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Transcriptomic analysis of maize developing earshoot, IBMRIL X IHP1 hybrid

Project description:Maize earshoot is a metabolic sink espcially related to nitrogen metabolism. Studies on the transcriptomic and metabolic changes occuring in earshoot can provide interesting answers about the nitrogen metabolic potential of the maize variety under study. Intermated B73 X Mo17 recombinant inbred lines (IBMRIL) belong to a predominantly used mapping population. Illinois high protein (IHP) line is a metabolic extreme with respect to nitrogen mebolism with around 30% seed protein. Developing earshoots from this genotype grown at nitrogen-deficient and nitrogen-sufficient conditions were sampled, processed and analyzed through microarray technology. IBMRIL population was crossed to IHP to find the allelic interaction related to nitrogen metabolism. Microarray experiment was conducted using the developing earshoots from IBMRIL X IHP1 lines that deffered in their grain yield significantly. Keywords: Yield response Technical replicates, dye swaps, technical replicates of dye swaps, no biological replicates

2010-02-03 | E-GEOD-17953 | biostudies-arrayexpress

Transcriptome analysis of 16 DAP seeds of Illinois protein lines.

Project description:Illinois protein lines (IPL) comprise lines known as Illinois high protein (IHP), Illinois low protein (ILP), Illinois reverse-low protein (IRLP) and Illinois reverse-high protein (IRHP) lines. These lines represent over a century old experiment containing lines that represent various levels of nitrogen metabolism potential as a function of their grain protein concentration. Microarray experiment was conducted using 16 DAP seeds from the above mentioned four IPLs. Keywords: Genotype response

2010-01-14 | GSE18006 | GEO

Genotype changes in 8DAP leaf

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Transcriptomic analysis of maize developing earshoot from tropical maize hybrid genotypes native to Nigeria

Project description:Tropical maize genotypes are well known for their traits of late flowering and higher biomass. Carbon/Nitrogen balance in these genotypes is significantly different from temperate genotypes. Microarray analysis of gene expression changes occuring in developing earshoots was done and results were compared with the temperate genotype microarray analysis, previously conducted. A low yielding variety was used as a reference against three high yielding varities. Keywords: Genotype response Technical replicates, dye swaps, technical replicates of dye swaps, no biological replicates

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