DNA methylation programming and reprogramming in primate embryonic stem cells

ABSTRACT: DNA methylation is an important epigenetic mechanism, affecting normal development and playing a key role in reprogramming epigenomes during stem cell derivation. Here we report on DNA methylation patterns in native monkey embryonic stem (ES) cells, fibroblasts and ES cells generated through somatic cell nuclear transfer (SCNT), identifying and comparing epigenome programming and reprogramming. We characterize hundreds of regions that are hyper- or hypo- methylated in fibroblasts compared to native ES cells and show that these are conserved in human cells and tissues. Remarkably, the vast majority of these regions are reprogrammed in SCNT ES cells, leading to almost perfect correlation between the epigenomic profiles of the native and reprogrammed lines. At least 58% of these changes are c

ORGANISM(S): Macaca mulatta

SUBMITTER: Amos Tanay 

PROVIDER: E-GEOD-17981 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress