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Arabidopsis lyrata small RNAs

ABSTRACT: Small RNA sequences from Arabidopsis lyrata leaves, as isolated from a single sample of rosette leaf tissue. These data were analyzed to 1) examine microRNA processing accuracy in A. lyrata and 2) to examine patterns of 24nt siRNA accumulation in A. lyrata. A single small RNA library from rosette leaf tissue was analyzed using an Illumina Genome Analyzer.

ORGANISM(S): Arabidopsis lyrata

SUBMITTER: Michael Axtell

PROVIDER: E-GEOD-18077 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Arabidopsis lyrata small RNAs: transient MIRNA and small interfering RNA loci within the Arabidopsis genus.

Ma Zhaorong Z Coruh Ceyda C Axtell Michael J MJ

The Plant cell 20100420 4

Twenty-one-nucleotide microRNAs (miRNAs) and 24-nucleotide Pol IV-dependent small interfering RNAs (p4-siRNAs) are the most abundant types of small RNAs in angiosperms. Some miRNAs are well conserved among different plant lineages, whereas others are less conserved, and it is not clear whether less-conserved miRNAs have the same functionality as the well conserved ones. p4-siRNAs are broadly produced in the Arabidopsis genome, sometimes from active hot spot loci, but it is unknown whether indivi ...[more]

PMID: 20407023

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Arabidopsis lyrata small RNAs

Project description:Small RNA sequences from Arabidopsis lyrata leaves, as isolated from a single sample of rosette leaf tissue. These data were analyzed to 1) examine microRNA processing accuracy in A. lyrata and 2) to examine patterns of 24nt siRNA accumulation in A. lyrata.

2010-03-01 | GSE18077 | GEO

Arabidopsis lyrata flowering tissues small RNAs

Project description:Small RNA sequences from Arabidopsis lyrata flowering tissues, as isolated from flowering tissues of two biological replicates. These data were analyzed to 1) discover new micoRNAs in A. lyrata 2) examine microRNA processing accuary in A. lyrata and 3) to examine patterns of 24nt siRNA accumulation in A. lyrata. Two small RNA libraries from flowering tissues of two biological replicates were analyzed using an Applied Biosystems SOLiD™ System

2010-04-22 | E-GEOD-20442 | biostudies-arrayexpress

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Arabidopsis thaliana and Arabidopsis lyrata degradomes

Project description:Deep sequencing of the 5' ends of uncapped, polyA-enriched mRNA from two biological replicate samples from Arabidopsis thaliana inflorescences, as well as two biological replicates of Arabidopsis lyrata inflorescences. These data were used to experimentally identify sliced microRNA targets from the two species. Two biological replicate samples of the 5' ends of uncapped, polyA+ RNAs from both A. thaliana and A. lyrata

2010-04-22 | E-GEOD-20451 | biostudies-arrayexpress

Transposable elements and small RNAs contribute to gene expression divergence between Arabidopsis thaliana and Arabidopsis lyrata [RNA-Seq]

Project description:Transposable elements (TEs) are often the primary determinant of genome size differences among eukaryotes. In plants, the proliferation of TEs is countered through epigenetic silencing mechanisms that prevent transposition. Recent studies using the model plant Arabidopsis thaliana have revealed that methylated TE insertions are often associated with reduced expression of nearby genes, and these insertions may be subject to purifying selection due to their effect on nearby genes. Less is known about the genome-wide patterns of epigenetic silencing of TEs in other plant species. Here, we compare the 24-nt siRNA complement from Arabidopsis thaliana and a closely related congener with a two- to three-fold higher TE copy number, A. lyrata. We show that TEs, and particularly siRNA-targeted TEs, are associated with reduced gene expression within both species and also with gene expression differences between orthologs. In addition, A. lyrata TEs are targeted by a lower fraction of uniquely matching siRNAs, which are associated with more effective silencing of TE expression. Overall, our results suggest that the efficacy of RNA-directed DNA methylation silencing is lower in A. lyrata, a finding that may shed light on the causes of differential TE proliferation among species. 4 A. lyrata mRNA-seq samples

2011-02-11 | E-GEOD-24571 | biostudies-arrayexpress

MIRNA Gene Evolution in Arabidopsis lyrata and Arabidopsis thaliana: Illumina sequencing

Project description:Deep sequencing of small RNA from three closely related brassicaceae A. thaliana, A. lyrata and Capsella rubella was done to systematically analyze the evolution of MIRNA genes and their targets. Small RNA were extracted from total RNA by size fractionation and converted to DNA amplicons by serial adaptor ligation to both ends followed by RT-PCR. DNA amplicons were sequenced using an Ilumina Genome Analyzer. Resulting sequences were computationally trimmed to remove 3' adaptor sequences. Raw data files (fastq) are unavailable for two of the samples (A.lyrata_flowers-stage1-12_rep1 and A.lyrata_flowers-stage1-12_rep2).

2010-05-14 | E-GEOD-20660 | biostudies-arrayexpress

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Project description:This SuperSeries is composed of the following subset Series: GSE24571: Transposable elements and small RNAs contribute to gene expression divergence between Arabidopsis thaliana and Arabidopsis lyrata [RNA-Seq] GSE38109: Natural variation in Arabidopsis thaliana transcriptomes Refer to individual Series

2012-05-21 | E-GEOD-38113 | biostudies-arrayexpress

Comparative transcriptome analysis in response to heat stress in Arabidopsis thaliana and Arabidopsis lyrata

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2015-08-01 | GSE69077 | GEO

Genome-wide profiling of H3K4me3, H3K27me3 H3K27me1 and 5mC enrichment and mRNA levels in young leaves of Arabidopsis lyrata

Project description:Chromatin, DNA and RNA were extracted from young A. alpina Pajares primary stem leaves. Chromatin and DNA methylation immunoprecipitation experiments were performed using commercially available antibodies and analyzed by Illumina sequencing (ChIP-seq and MeDIP-seq). Transcriptome data were generated by RNA-seq. ChIP-seq analysis of H3K4me3, H3K27me3 and H3K27me1 enrichment profiles and MeDIP-seq analysis of 5mC enrichment profiles in 2 biological replicates. RNA-seq analysis of mRNA levels in 1 biological replicate.

2015-02-03 | E-GEOD-54727 | biostudies-arrayexpress

Comparative transcriptome analysis in response to heat stress in Arabidopsis thaliana and Arabidopsis lyrata

2015-08-01 | E-GEOD-69077 | biostudies-arrayexpress