Unknown,Transcriptomics,Genomics,Proteomics

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Expression from C. elegans L1 animals


ABSTRACT: Nutrient-driven O-GlcNAcylation of key components of the transcription machinery may epigenetically modulate gene expression in metazoans. Knockouts of the O-GlcNAc cycling enzymes in C. elegans are viable and fertile, allowing a global analysis of the impact of GlcNAcylation. Whole genome transcriptional profiling of the O-GlcNAc cycling mutants confirmed dramatic deregulation of genes in these key pathways. As predicted, the O-GlcNAc cycling mutants show phenotypically altered lifespan and susceptibility to UV stress. L1 larvae were synchronized by starvation for two days after hatching in sterile buffer. Wild type and mutant animals were collected and total RNA isolated for gene expression analysis

ORGANISM(S): Caenorhabditis elegans

SUBMITTER: Michael Krause 

PROVIDER: E-GEOD-18130 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Dynamic O-GlcNAc cycling at promoters of Caenorhabditis elegans genes regulating longevity, stress, and immunity.

Love Dona C DC   Ghosh Salil S   Mondoux Michelle A MA   Fukushige Tetsunari T   Wang Peng P   Wilson Mark A MA   Iser Wendy B WB   Wolkow Catherine A CA   Krause Michael W MW   Hanover John A JA  

Proceedings of the National Academy of Sciences of the United States of America 20100405 16


Nutrient-driven O-GlcNAcylation of key components of the transcription machinery may epigenetically modulate gene expression in metazoans. The global effects of GlcNAcylation on transcription can be addressed directly in C. elegans because knockouts of the O-GlcNAc cycling enzymes are viable and fertile. Using anti-O-GlcNAc ChIP-on-chip whole-genome tiling arrays on wild-type and mutant strains, we detected over 800 promoters where O-GlcNAc cycling occurs, including microRNA loci and multigene o  ...[more]

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