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Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling by array of uteruses from immature Alpk:APfCD-1 mice treated with arachis oil vehicle (AO), 17beta-estradiol (E2), or genistein (GEN) to study the effects of E2 and GEN on gene expression.

ABSTRACT: Immature (19/20 days of age) Alpk:APfCD-1 mice were treated with arachis oil (AO) vehicle, 0.4mg/kg 17beta-estradiol (E2), or 250mg/kg genistein (GEN), via a single subcutaneous injection, and sacrificed at 1hr, 2hr, 4hr, 8hr, 24hr, 48hr, 72hr post dose.

ORGANISM(S): Mus musculus

SUBMITTER: Fei Ling Lim

PROVIDER: E-GEOD-1819 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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The need to decide if all estrogens are intrinsically similar.

Moggs Jonathan G JG Ashby John J Tinwell Helen H Lim Fei Ling FL Moore David J DJ Kimber Ian I Orphanides George G

Environmental health perspectives 20040801 11

We used gene expression profiling to investigate whether the molecular effects induced by estrogens of different provenance are intrinsically similar. In this article we show that the physiologic estrogen 17-beta-estradiol, the phytoestrogen genistein, and the synthetic estrogen diethylstilbestrol alter the expression of the same 179 genes in the intact immature mouse uterus under conditions where each chemical has produced an equivalent gravimetric and histologic uterotrophic effect, using the ...[more]

PMID: 15289156

Similar Datasets

Project description:Immature (19/20 days of age) Alpk:APfCD-1 mice were treated with arachis oil (AO) vehicle, 0.4mg/kg 17beta-estradiol (E2), or 250mg/kg genistein (GEN), via a single subcutaneous injection, and sacrificed at 1hr, 2hr, 4hr, 8hr, 24hr, 48hr, 72hr post dose. Keywords: other

2004-10-06 | GSE1819 | GEO

Transcription profiling of uteruses from Alpk:APfCD-1 mice treated with arachis oil (AO) vehicle, 17beta-estradiol (E2), diethylstilbestrol (DES), or genistein (GEN) to study the effects of E2, GEN, and DES on gene transcription.

Project description:Three independent replicates of 4 groups of immature (19/20 days of age) Alpk:APfCD-1 mice were treated with arachis oil (AO) vehicle, 2.5ug/kg 17beta-estradiol (E2), 2ug/kg diethylstilbestrol (DES), or 50mg/kg genistein (GEN), via 3 daily subcutaneous injection, and sacrificed 72hr after initial dose.

2007-12-13 | E-GEOD-1839 | biostudies-arrayexpress

Transcription profiling of uteri from mice treated with 17beta-estradiol for different durations to study the molecular mechanism for estrogen induced uterine growth.

2007-09-22 | E-GEOD-2195 | biostudies-arrayexpress

Equivalence of biologic responses induced in the mouse uterus by E2, GEN, and DES

2004-10-14 | GSE1839 | GEO

Phenotypic Anchoring of Gene Expression Changes during Estrogen-Induced Uterine Growth

Project description:Immature (19/20 days of age) Alpk:APfCD-1 mice were treated with arachis oil (AO) vehicle or 0.4mg/kg 17beta-estradiol (E2), via a single subcutaneous injection, and sacrificed at 1hr, 2hr, 4hr, 8hr, 24hr, 48hr, 72hr post dose. Keywords = estrogen Keywords = gene expression Keywords = microarray Keywords = phenotypic anchoring Keywords = uterus Keywords: time-course

2005-01-28 | GSE2195 | GEO

Mouse cardiac tissue: Vehicle treated control vs. 25mg/kg DMNQ

2010-08-06 | GSE17915 | GEO

Mouse cardiac tissue, polysomes and monosomes: Vehicle treated control vs. 25mg/kg DMNQ

Project description:Translational profiling of mouse cardiac tissue treated with 25mg/kg DMNQ in 10 ml/kg arachis oil over an acute time course (0.5-120 hours) compared to time matched control animals treated with 10ml/kg saline

2010-08-06 | GSE17830 | GEO

Mouse cardiac tissue: Vehicle treated control vs. 25mg/kg DMNQ

Project description:Transcriptional profiling of mouse cardiac tissue treated with 25mg/kg DMNQ in 10 ml/kg arachis oil over an acute time course (5 minutes-120 hours) compared to time matached control animals treated with 10ml/kg arachis oil Two colour microarrays with time matched controls against 25mg/kg DMNQ cardiac tissue. Time points studied were 5, 10, 15, 20, 30, 45 minutes and 1, 1.5, 2, 5, 12, 24, 120 hours following dosing, biological replicates n=>2 independent animals at each time point, technical replicate n>1 with reverse labelling at each time point. One array printed onto two slides (A and B), one replicate per array.

2010-08-06 | E-GEOD-17915 | biostudies-arrayexpress

Dissection of corticotroph transcriptome in a mouse model of glucocorticoid-induced suppression of the HPA axis

Project description:The effect on the transcriptome of dexamethasone (0.4mg/kg) treatment, and recovery 1 and 4 weeks following withdrawal was examined in corticotrophs FACS isolated from POMC-GFP mice. We show dexamethasone has persistent effects on the corticotroph transcriptome, some lasting up to 4 weeks.

2022-01-03 | GSE165026 | GEO

Effects of neonatal dosing of SERMs on CD1 mouse uterine gene expression at 3 months

Project description:Tamoxifen, a selective estrogen receptor modulator (SERM), is an effective treatment for breast cancers. In the CD-1 mouse model, neonatal oral dosing with tamoxifen leads to the development of adenomyosis. Both 4-hydroxyestradiol and tamoxifen can form DNA-reactive metabolites and may be involved in carcinogenesis of the uterus. After comparing the uterotrophic response of several SERMs the maximal uterotrophic doses of estradiol (100mg/kg) 4-hydroxyestradiol (385mg/kg) and tamoxifen (250mg/kg) were determined. Maximal uterotrophic doses were given orally to newborn CD-1 mice on days 1 â 4 after birth and gene and pathological changes examined in the uterus at 3 months after dosing. ERKOa knockout mice were dosed orally with tamoxifen (1mg/Kg) on days 1 â 4 after birth and uterine gene expression compared with CD-1 mice. Dosing groups: Estradiol (E2): 100mg/Kg 4-hydroxyestradiol (4OHE2): 385mg/Kg Tamoxifen : 250mg/Kg ERKO mice dosed with tamoxifen (1mg/Kg) 4 dosed animals and 4 controls for each treatment group. Uteri removed at 3 months after dosing and total RNA extracted. Controls were pooled. RNA labelling, hybridisation and analysis of fluorescence was carried out as described by Turton et al (2001). Cy3/Cy5 dye swap labelling was carried out on samples from each animal. Reference: Turton NJ et. al. (Oncogene (2001) 20, 1300-1306

2010-06-30 | E-GEOD-3625 | biostudies-arrayexpress

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